Effects of dl-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability.

Abstract

The present study was conducted in a one-factorial arrangement to determine the effects of dl-2-hydroxy-4-methylthiobutyrate (dl-HMTB) on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability. Barrows (n 6; aged 35 d; weight 8.6 kg), implanted with arterial, portal, mesenteric and gastric catheters, were fed a diet containing dl-methionine (dl-MET) or dl-HMTB once hourly and infused intramesenterically with 1 % p-aminohippurate and intragastrically with [1-13C]methionine at 7.0 mumol/kg body weight per h. Arterial and portal blood samples were taken at hourly intervals until 6 h of tracer infusion and pigs was then killed for collection of muscle, intestine, liver and kidney samples. The net portal appearance of methionine, expressed as the fraction of ingested directly available l-methionine, was higher (P < 0.05) in the dl-HMTB than in the dl-MET diet, and there was no difference (P = 0.26) in the fractional portal balance of [1-13C]methionine between the diets. [1-13C]methionine enrichment (tracer:tracee ratio; mol/100 mol amino acid) in the jejunum, arterial and portal plasma, liver, kidney and muscle was also not different (P>0.05) between the groups. Over the 6 h period after the start of feeding, the average concentration of citrulline both in the arterial and portal plasma was higher (P < 0.05) in the dl-HMTB than in the dl-MET group, and arterial plasma ornithine and taurine concentration was also higher (P < 0.05) in the dl-HMTB than in the dl-MET group. However, plasma urea concentration both in the arterial and portal vein was lower (P < 0.05) in the dl-HMTB than in the dl-MET group. These results suggested that the potential difference in the first-pass use of methionine by the intestine between the dl-HMTB and dl-MET diets might affect intestinal and systemic metabolism of other amino acids, which may provide new important insights into nutritional efficiency of different methionine sources.

• Authors: Zhengfeng Fang, Feiruo Huang, Jie Luo, Hongkui Wei, Libao Ma, Siwen Jiang, Jian Peng
• Journal: The British journal of nutrition (Br J Nutr) Vol. 103 Issue 5 Pg. 643-51 (Mar 2010) ISSN: 1475-2662 [Electronic] England
• PMID: 20064283 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Dietary Proteins
• alpha-hydroxy-gamma-methylmercaptobutyric acid
• Urea
• Methionine
• p-Aminohippuric Acid

Topics

• Amino Acids (blood)
• Animals
• Diet
• Dietary Proteins (metabolism)
• Intestinal Absorption (physiology)
• Intestine, Small (metabolism)
• Kidney (metabolism)
• Liver (metabolism)
• Methionine (analogs & derivatives, blood, metabolism)
• Muscles (metabolism)
• Portal System (metabolism)
• Swine
• Urea (blood)
• p-Aminohippuric Acid