Abstract | OBJECTIVE: To evaluate the effects of selective P receptor (PR) modulator CDB4124 on cell proliferation and apoptosis in cultured human uterine leiomyoma smooth muscle (LSM) cells and control myometrial smooth muscle (MSM) cells in matched uteri. DESIGN: Laboratory research. SETTING: Academic medical center. PATIENT(S): INTERVENTION(S): Treatment of primary LSM and MSM cells with CDB4124 (10(-8)-10(-6) M) or vehicle for 24, 48, or 72 hours. MAIN OUTCOME MEASURE(S): RESULT(S): Treatment with CDB4124 significantly decreased levels of the proliferation marker proliferating cell nuclear antigen, the number of viable LSM cells, and the antiapoptotic protein Bcl-2. On the other hand, treatment with CDB4124 increased levels of the apoptosis marker cleaved polyadenosine 5'-diphosphate-ribose polymerase and the tumor suppressor Krüppel-like transcription factor 11 in a dose- and time-dependent manner in LSM cells. In matched MSM cells, however, CDB4124 did not affect cell proliferation or apoptosis. CONCLUSION(S):
CDB4124 selectively inhibits proliferation and induces apoptosis in LSM but not in MSM cells.
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Authors | Xia Luo, Ping Yin, John S Coon V, You-Hong Cheng, Ronald D Wiehle, Serdar E Bulun |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 93
Issue 8
Pg. 2668-73
(May 15 2010)
ISSN: 1556-5653 [Electronic] United States |
PMID | 20056218
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Apoptosis Regulatory Proteins
- Cell Cycle Proteins
- KLF11 protein, human
- Norpregnadienes
- Proliferating Cell Nuclear Antigen
- Proto-Oncogene Proteins c-bcl-2
- Receptors, Progesterone
- Repressor Proteins
- telapristone acetate
- Poly(ADP-ribose) Polymerases
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Topics |
- Adult
- Apoptosis
(drug effects)
- Apoptosis Regulatory Proteins
- Cell Cycle Proteins
(biosynthesis)
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Cells, Cultured
- Female
- Humans
- Leiomyoma
(pathology)
- Middle Aged
- Myometrium
(drug effects)
- Norpregnadienes
(pharmacology)
- Poly(ADP-ribose) Polymerases
(drug effects, metabolism)
- Proliferating Cell Nuclear Antigen
(biosynthesis)
- Proto-Oncogene Proteins c-bcl-2
- Receptors, Progesterone
(drug effects)
- Repressor Proteins
(biosynthesis)
- Uterine Neoplasms
(pathology)
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