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Fusion of HBsAg and prime/boosting augment Th1 and CTL responses to HCV polytope DNA vaccine.

Abstract
Correlation of hepatitis C virus (HCV) spontaneous resolution with Th1 and CD8(+)CTL responses during natural infection implies the potentiality of poly-CTL-epitopic HCV vaccines. We recently reported in silico design and construction of DNA vaccines (pcPOL-plasmids) harboring HCV CTL epitopes. Herein, we provide data of mice immunization by pcPOL, (encoding; core(132-142) [C], E2(405-414) [E(4)], E2(614-622) [E(6)] and NS3(1406-1415) [N] CD8(+)CTL epitopes as CE(4)E(6)N polytope) and its HBsAg-fused counterpart (pcHPOL), compared to the adjuvant-formulated (Montanide+CpG) CE(4)E(6)N synthetic-peptide immunization. All vaccinated groups developed different levels of cellular responses, however, only the pcHPOL-immunized mice elicited strong CTLs and IFN-gamma-secreting cells that were further augmented towards a Th1 response and partial tumor protection by DNA-prime/peptide-boosting regimen. Priming with HBsAg alone could not afford its augmenting effect indicating the importance of priming by polytope itself. Hence, fusion of immunocarriers like HBsAg conjoined with DNA-prime/peptide-boost immunization regimen seems a strategy to enhance the epitope-specific immune responses towards poly-CTL-epitopic vaccines.
AuthorsArash Memarnejadian, Farzin Roohvand
JournalCellular immunology (Cell Immunol) Vol. 261 Issue 2 Pg. 93-8 ( 2010) ISSN: 1090-2163 [Electronic] Netherlands
PMID20056196 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright2009 Elsevier Inc. All rights reserved.
Chemical References
  • Epitopes
  • Hepatitis B Surface Antigens
  • Vaccines, DNA
  • Viral Hepatitis Vaccines
Topics
  • Animals
  • Epitopes (genetics, immunology)
  • Female
  • Hepacivirus (immunology)
  • Hepatitis B Surface Antigens (genetics, immunology)
  • Hepatitis C (immunology, prevention & control)
  • Immunization, Secondary
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocyte Subsets (immunology)
  • T-Lymphocytes, Cytotoxic (immunology)
  • Th1 Cells (immunology)
  • Vaccines, DNA (immunology)
  • Viral Hepatitis Vaccines (immunology)

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