Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells.

Abstract

Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Ialpha and Ibeta and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Ialpha and Ibeta led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-gamma, tumor necrosis factor-alpha, granulocyte-colony stimulating factor or transforming growth factor-beta1. The elevated IL-6 expression seen in REG Ialpha transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology.

• Authors: Shuetsu Usami, Satoru Motoyama, Souichi Koyota, Jingshu Wang, Kaori Hayashi-Shibuya, Kiyotomi Maruyama, Naoko Takahashi, Hajime Saito, Yoshihiro Minamiya, Shin Takasawa, Jun-Ichi Ogawa, Toshihiro Sugiyama
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 392 Issue 1 Pg. 4-8 (Jan 29 2010) ISSN: 1090-2104 [Electronic] United States
• PMID: 20056108 (Publication Type: Journal Article)
• Copyright: Copyright (c) 2009 Elsevier Inc. All rights reserved.

Chemical References

• Interleukin-6
• Lithostathine
• REG1A protein, human
• REG1B protein, human

Topics

• Cell Line, Tumor
• Esophageal Neoplasms (metabolism)
• Humans
• Interleukin-6 (biosynthesis)
• Lithostathine (genetics, metabolism)
• Neoplasms, Squamous Cell (metabolism)
• Transfection