Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate.

Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage.
Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database.
A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and alpha-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples.
The loss of vitamin D-binding protein, haptoglobin and alpha-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.
AuthorsHsien-Yi Wang, Yu-Feng Tian, Chih-Chiang Chien, Wei-Chih Kan, Pao-Chi Liao, Hsin-Yi Wu, Shih-Bin Su, Ching-Yih Lin
JournalNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (Nephrol Dial Transplant) Vol. 25 Issue 6 Pg. 1955-63 (Jun 2010) ISSN: 1460-2385 [Electronic] England
PMID20054029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers
  • Dialysis Solutions
  • HP protein, human
  • Haptoglobins
  • IgK
  • Immunoglobulins
  • Proteome
  • Vitamin D-Binding Protein
  • alpha-Macroglobulins
  • Complement C4a
  • Adult
  • Aged
  • Ascitic Fluid (chemistry)
  • Biomarkers (analysis)
  • Blotting, Western
  • Complement C4a (analysis)
  • Diabetic Nephropathies (metabolism, therapy)
  • Dialysis Solutions (chemistry)
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • Haptoglobins (analysis)
  • Humans
  • Immunoglobulins (analysis)
  • Male
  • Middle Aged
  • Peritoneal Dialysis (adverse effects)
  • Peritoneum (chemistry, injuries)
  • Proteome (analysis)
  • Proteomics
  • Tandem Mass Spectrometry
  • Vitamin D-Binding Protein (analysis)
  • alpha-Macroglobulins (analysis)

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