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Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate.

AbstractBACKGROUND:
Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage.
METHODS:
Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database.
RESULTS:
A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and alpha-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples.
CONCLUSION:
The loss of vitamin D-binding protein, haptoglobin and alpha-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.
AuthorsHsien-Yi Wang, Yu-Feng Tian, Chih-Chiang Chien, Wei-Chih Kan, Pao-Chi Liao, Hsin-Yi Wu, Shih-Bin Su, Ching-Yih Lin
JournalNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (Nephrol Dial Transplant) Vol. 25 Issue 6 Pg. 1955-63 (Jun 2010) ISSN: 1460-2385 [Electronic] England
PMID20054029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers
  • Dialysis Solutions
  • HP protein, human
  • Haptoglobins
  • IgK
  • Immunoglobulins
  • Proteome
  • Vitamin D-Binding Protein
  • alpha-Macroglobulins
  • Complement C4a
Topics
  • Adult
  • Aged
  • Ascitic Fluid (chemistry)
  • Biomarkers (analysis)
  • Blotting, Western
  • Complement C4a (analysis)
  • Diabetic Nephropathies (metabolism, therapy)
  • Dialysis Solutions (chemistry)
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • Haptoglobins (analysis)
  • Humans
  • Immunoglobulins (analysis)
  • Male
  • Middle Aged
  • Peritoneal Dialysis (adverse effects)
  • Peritoneum (chemistry, injuries)
  • Proteome (analysis)
  • Proteomics
  • Tandem Mass Spectrometry
  • Vitamin D-Binding Protein (analysis)
  • alpha-Macroglobulins (analysis)

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