HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effect of the C3a-receptor antagonist SB 290157 on anti-OVA polyclonal antibody-induced arthritis.

Abstract
It was investigated whether the C3a-receptor antagonist (C3aRA) SB 290157 was involved in the suppression of anti-OVA pAb-induced arthritis because it is well known that anaphylatoxin C3a plays a crucial role in the development of an effective inflammatory response during complement activation. Anti-OVA pAb-induced arthritis was induced in DBA/1J mice by administration of anti-OVA pAb 0.5 h prior to intra-articular (i.a.) injection of OVA (0 h). Two peaks of joint swelling were observed at 0.5 and 3 h. The role of C3aRA in arthritis was investigated by injection of SB 290157 at concentrations of 10 and 30 mg/kg at 0 and 2 h. The antagonist was able to reduce joint swelling only at 3 h, and about 50% inhibition of joint swelling was observed with the concentration of 30 mg/kg. The C3 level was significantly decreased at 3 h compared with naïve mice showing complement consumption. Furthermore, the C3 activation was observed and increased corresponding to the graded concentration of anti-OVA pAb. The results also revealed that the C3aRA was able to reduce the expression of IL-1beta in synovial tissue. Taken together, the results suggested that C3aRA may be effective in the inhibition of arthritis.
AuthorsPilaiwanwadee Hutamekalin, Kohei Takeda, Mitsuhiro Tani, Yuko Tsuga, Naoki Ogawa, Nobuaki Mizutani, Shin Yoshino
JournalJournal of pharmacological sciences (J Pharmacol Sci) Vol. 112 Issue 1 Pg. 56-63 ( 2010) ISSN: 1347-8648 [Electronic] Japan
PMID20051658 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies
  • Benzhydryl Compounds
  • Receptors, Complement
  • SB 290157
  • Complement C3a
  • Arginine
Topics
  • Animals
  • Antibodies (toxicity)
  • Arginine (analogs & derivatives, pharmacology, therapeutic use)
  • Arthritis, Experimental (immunology, metabolism, prevention & control)
  • Benzhydryl Compounds (pharmacology, therapeutic use)
  • Complement C3a (antagonists & inhibitors, metabolism)
  • Male
  • Mice
  • Mice, Inbred DBA
  • Ovum (immunology)
  • Rats
  • Rats, Inbred Lew
  • Receptors, Complement (antagonists & inhibitors, immunology, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: