Cationic amphiphile with shikimic acid headgroup shows more systemic promise than its mannosyl analogue as DNA vaccine carrier in dendritic cell based genetic immunization.

Abstract

Mannosylated cationic vectors have been previously used for delivering DNA vaccines to antigen presenting cells (APCs) via mannose receptors expressed on the cell surface of APCs. Here we show that cationic amphiphiles containing mannose-mimicking quinic acid and shikimic acid headgroups deliver genes to APCs via mannose receptor. Cationic amphiphile with shikimic acid headgroup was more efficacious than its mannosyl counterpart in combating mouse tumor growth by dendritic cell (the most professional APC) based genetic immunization.

• Authors: Ramishetti Srinivas, Priya P Karmali, Dipankar Pramanik, Arup Garu, Yenugonda Venkata Mahidhar, Bharat K Majeti, Sistla Ramakrishna, Gunda Srinivas, Arabinda Chaudhuri
• Journal: Journal of medicinal chemistry (J Med Chem) Vol. 53 Issue 3 Pg. 1387-91 (Feb 11 2010) ISSN: 1520-4804 [Electronic] United States
• PMID: 20050668 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cations
• Lectins, C-Type
• Liposomes
• Mannose Receptor
• Mannose-Binding Lectins
• Receptors, Cell Surface
• Vaccines, DNA
• Shikimic Acid
• Mannose

Topics

• Animals
• Antigen-Presenting Cells
• Cations (chemistry)
• Cells, Cultured
• Dendritic Cells (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Female
• Immunization
• Lectins, C-Type (metabolism)
• Liposomes
• Mannose (chemical synthesis, chemistry, pharmacology)
• Mannose Receptor
• Mannose-Binding Lectins (metabolism)
• Mass Spectrometry
• Melanoma, Experimental (drug therapy)
• Mice
• Mice, Inbred C57BL
• NIH 3T3 Cells
• Receptors, Cell Surface (metabolism)
• Shikimic Acid (chemistry)
• Vaccines, DNA (administration & dosage)