Semisynthetic latrunculin derivatives as inhibitors of metastatic breast cancer: biological evaluations, preliminary structure-activity relationship and molecular modeling studies.

Abstract	The microfilament cytoskeleton protein actin plays an important role in cell biology and affects cytokinesis, morphogenesis, and cell migration. These functions usually fail and become abnormal in cancer cells. The marine-derived macrolides latrunculins A and B, from the Red Sea sponge Negombata magnifica, are known to reversibly bind actin monomers, forming 1:1 stoichiometric complexes with G-actin, disrupting its polymerization. To identify novel therapeutic agents for effective treatment of metastatic breast cancer, several semisynthetic derivatives of latrunculin A with diverse steric, electrostatic, and hydrogen bond donor and acceptor properties were rationally prepared. Analogues were designed to modulate the binding affinity toward G-actin. Examples of these reactions are esterification, acetylation, and N-alkylation. Semisynthetic latrunculins were then tested for their ability to inhibit pyrene-conjugated actin polymerization, and subsequently assayed for their antiproliferative and anti-invasive properties against MCF7 and MDA-MB-231 cells using MTT and invasion assays, respectively.
Authors	Mohammad A Khanfar, Diaa T A Youssef, Khalid A El Sayed
Journal	ChemMedChem (ChemMedChem) Vol. 5 Issue 2 Pg. 274-85 (Feb 01 2010) ISSN: 1860-7187 [Electronic] Germany
PMID	20043312 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References	Actins Antineoplastic Agents Bridged Bicyclo Compounds, Heterocyclic Thiazolidines latrunculin A
Topics	Actins (antagonists & inhibitors, metabolism) Animals Antineoplastic Agents (chemical synthesis, chemistry, pharmacology) Binding Sites Breast Neoplasms (drug therapy, pathology) Bridged Bicyclo Compounds, Heterocyclic (chemical synthesis, chemistry, pharmacology) Cell Line, Tumor Computer Simulation Crystallography, X-Ray Female Humans Hydrogen Bonding Models, Chemical Models, Molecular Neoplasm Metastasis Structure-Activity Relationship Thiazolidines (chemical synthesis, chemistry, pharmacology)

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