Specific nucleic acid detection using photophysical properties of quantum dot probes.

Abstract

In the present study, we report a novel separation-free method to detect and quantify avian influenza virus A (H5N1) nucleic acid without amplification, based on the alteration of photophysical parameters of quantum dot (QD) probes after hybridization with specific complementary target DNA. The target DNA was quantified in a custom-made portable device by simultaneously measuring lifetime and quenching of the QD probes. QD probes (25-mer) showed a 30% lifetime reduction and 40% fluorescence quenching when hybridized with complementary 25-mer target DNA. In comparison with a conventional QD-based assay, this assay provides a simple quantitation of nucleic acids with a single labeling step.

• Authors: Sun Hee Lim, Philippe Buchy, Sek Mardy, Moon Sik Kang, Alexey Dan Chin Yu
• Journal: Analytical chemistry (Anal Chem) Vol. 82 Issue 3 Pg. 886-91 (Feb 01 2010) ISSN: 1520-6882 [Electronic] United States
• PMID: 20041684 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• Oligonucleotide Probes
• DNA

Topics

• DNA (analysis)
• DNA, Viral (analysis)
• Influenza A Virus, H5N1 Subtype (genetics, isolation & purification)
• Kinetics
• Nucleic Acid Hybridization (methods)
• Oligonucleotide Probes (chemistry)
• Quantum Dots