Effect of taxifolin glycoside on atopic dermatitis-like skin lesions in NC/Nga mice.

Abstract

Increased levels of eosinphils, IgE, IL-4, 5, and 13 and pro-inflammatory factors (COX-2, iNOS) are observed in patients with atopic dermatitis (AD). Taxifolin 3-O-beta-D-glucopyranoside (TAX) from the roots of Rhododendron mucronulatum (RM) was examined to determine whether its immunomodulatory effect was applicable for treating atopic dermatitis.A total of 7 groups of NC/Nga mice with AD were treated by topical application or intraperitoneal injection of TAX for 4 weeks. Follow-up evaluations were done to assess the changes in clinical observations, eosinophil counts, and levels of IgE, cytokines, COX-2 and iNOS.In the clinical observation during the experimental period, TAX treatment significantly reduced the severity of AD-like lesions induced in NC/Nga mice. Eosinophil and IgE levels decreased after treatment of the animals with TAX. TAX may thus be associated with improvement of eosinophil-related allergic diseases. The expression of cytokines (IL-4, 5 and 13) was significantly inhibited in the TAX-treated group, suggesting that TAX might play an immunoregulatory role associated with AD. In RT-PCR, iNOS and COX-2 expression levels were reduced in the TAX-treated group. In western blotting, the expression levels of iNOS and COX-2 were also reduced in the TAX-treated group.These findings suggest that TAX is effective for the treatment of AD by preventing the production of inflammatory cytokines and by reducing skin inflammation.

• Authors: Ji Young Ahn, Sun Eun Choi, Mi Sook Jeong, Kwan Hee Park, Nam Ju Moon, Seong Soo Joo, Chung Soo Lee, Young Wook Choi, Kapsok Li, Mi-Kyung Lee, Min Won Lee, Seong Jun Seo
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 24 Issue 7 Pg. 1071-7 (Jul 2010) ISSN: 1099-1573 [Electronic] England
• PMID: 20041431 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Cyclopropanes
• Cytokines
• Glycosides
• Plant Extracts
• Immunoglobulin E
• Quercetin
• taxifolin
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• diphenylcyclopropenone

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Cyclooxygenase 2 (analysis)
• Cyclopropanes
• Cytokines (blood)
• Dermatitis, Atopic (drug therapy)
• Disease Models, Animal
• Eosinophils (immunology)
• Female
• Glycosides (pharmacology)
• Immunoglobulin E (blood)
• Inflammation (drug therapy, pathology)
• Mice
• Nitric Oxide Synthase Type II (analysis)
• Plant Extracts (pharmacology)
• Plant Roots (chemistry)
• Quercetin (analogs & derivatives, pharmacology)
• Rhododendron (chemistry)
• Skin (drug effects, pathology)