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Liquid chromatography tandem mass spectrometry applied to quantitation of the organophosphorus nerve agent VX in microdialysates from blood probes.

Abstract
VX (O-ethyl-S-[2(di-isopropylamino)ethyl] methylphosphonothiolate) is a low volatility organophosphorus (OP) nerve agent and therefore the most likely route of exposure is via percutaneous absorption. Microdialysis has been used as a tool to study percutaneous poisoning by VX in the anesthetised guinea pig. A liquid chromatography tandem mass spectrometry (LC-MS-MS) method using positive electrospray ionisation (ESI) was used to quantitate VX in microdialysate samples collected from microdialysis probes, implanted into a blood vessel of anesthetised guinea pigs. The method resulted from modification of a LC-MS-MS method previously developed for the analysis of dermal microdialysates. Modification increased the sensitivity of the method, allowing quantitation of the trace levels of VX in blood microdialysates, over the range 0.002-1 ng/ml, with linear calibration. Quantitative results have been used to determine the time course of VX concentrations in the blood of guinea pigs following percutaneous poisoning.
AuthorsS J Stubbs, R W Read
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 878 Issue 17-18 Pg. 1253-6 (May 15 2010) ISSN: 1873-376X [Electronic] Netherlands
PMID20034857 (Publication Type: Journal Article)
CopyrightCrown Copyright (c) 2009. Published by Elsevier B.V. All rights reserved.
Chemical References
  • Chemical Warfare Agents
  • Organothiophosphorus Compounds
  • VX
Topics
  • Administration, Cutaneous
  • Animals
  • Chemical Warfare Agents (analysis, pharmacokinetics)
  • Chromatography, Liquid (methods)
  • Dialysis
  • Drug Stability
  • Guinea Pigs
  • Linear Models
  • Organothiophosphorus Compounds (administration & dosage, blood, pharmacokinetics)
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry (methods)

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