Abstract |
Aloe-emodin, one of the anthraquinones, has been shown to have anticancer activity in different kinds of human cancer cell lines. Therefore, the purpose of this study was to investigate the anti- cancer effect of aloe-emodin on human tongue squamous carcinoma SCC-4 cells. The results indicated that aloe-emodin induced cell death through S-phase arrest and apoptosis in a dose- and time-dependent manner. Treatment with 30 microM of aloe-emodin led to S-phase arrest through promoted p53, p21 and p27, but inhibited cyclin A, E, thymidylate synthase and Cdc25A levels. Aloe-emodin promoted the release of apoptosis-inducing factor (AIF), endonuclease G (Endo G), pro-caspase-9 and cytochrome c from the mitochondria via a loss of the mitochondrial membrane potential (DeltaPsi(m)) which was associated with a increase in the ratio of B-cell lymphoma 2-associated X protein (Bax)/ B cell lymphoma/ leukemia-2 (Bcl-2) and activation of caspase-9 and -3. The free radical scavenger N-acetylcysteine (NAC) and caspase inhibitors markedly blocked aloe-emodin-induced apoptosis. Aloe-emodin thus induced apoptosis in the SCC-4 cells through the Fas/ death-receptor, mitochondria and caspase cascade. Aloe-emodin could be a novel chemotherapeutic drug candidate for the treatment of human tongue squamous cancer in the future.
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Authors | Tsan-Hung Chiu, Wan-Wen Lai, Te-Chun Hsia, Jai-Sing Yang, Tung-Yuan Lai, Ping-Ping Wu, Chia-Yu Ma, Chin-Chung Yeh, Chin-Chin Ho, Hsu-Feng Lu, W Gibson Wood, Jing-Gung Chung |
Journal | Anticancer research
(Anticancer Res)
Vol. 29
Issue 11
Pg. 4503-11
(Nov 2009)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 20032398
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anthraquinones
- Antineoplastic Agents
- Isoenzymes
- Reactive Oxygen Species
- aloe emodin
- Endodeoxyribonucleases
- endonuclease G
- Caspases
- Calcium
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Topics |
- Anthraquinones
(pharmacology)
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Calcium
(metabolism)
- Carcinoma, Squamous Cell
(drug therapy, metabolism, pathology)
- Caspases
(metabolism)
- Cell Line, Tumor
- Endodeoxyribonucleases
(metabolism)
- Humans
- Isoenzymes
(metabolism)
- Membrane Potential, Mitochondrial
(drug effects)
- Mitochondria
(drug effects, enzymology)
- Reactive Oxygen Species
(metabolism)
- S Phase
(drug effects)
- Tongue Neoplasms
(drug therapy, metabolism, pathology)
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