[Influence of AML1 and AML1-ETO on transcriptional regulation of nucb2 gene].

Abstract	This study was purposed to investigate the effect of AML1-ETO fusion protein resulted from hematopoietic transcription factor (AML1) and acute myeloid leukemia M(2b)(AML-M(2b)) on transcription activity of nucleobindin 2 (nucb2) promoter, and to explore the role of AML1-ETO in molecular pathogenesis of AML-M(2b). The real-time RT-PCR was used to study the regulation of AML1-ETO on nucb2 at transcription level in AML1-ETO inducible leukemia cell line, the chromatin immunoprecipitation (ChIP)-based qPCR was used to investigate the direct in vivo interaction between the AML1, AML1-ETO and nucb2 promoter in AML1-ETO positive leukemia cell line, the luciferase report gene assay was used to detect the regulation of AML1, AML1-ETO on the transcription activity of nucb2 promoter. The results showed that the expression level of nucb2 was reduced with the increase of AML1-ETO. The promoter of nucb2 could be bound by both AML1 and AML1-ETO. The promoter of nucb2 was trans-repressed by AML1 and AML1-ETO respectively. It is concluded that the nucb2 is the direct target gene of AML1 and AML1-ETO, the transcription regulation of AML1, AML1-ETO on nucb2 is carried out via repressing its promoter activity.
Authors	Bei Zou, Wen Jin, Jing Li, Jian-Tao Shi, Ji Zhang, Kan-Kan Wang
Journal	Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 17 Issue 6 Pg. 1482-6 (Dec 2009) ISSN: 1009-2137 [Print] China
PMID	20030931 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	AML1-ETO fusion protein, human Calcium-Binding Proteins Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins NUCB2 protein, human Nerve Tissue Proteins Nucleobindins Oncogene Proteins, Fusion RUNX1 Translocation Partner 1 Protein Transcription Factors
Topics	Calcium-Binding Proteins (genetics) Cell Line, Tumor Core Binding Factor Alpha 2 Subunit (genetics) DNA-Binding Proteins (genetics) Gene Expression Regulation, Leukemic Humans Nerve Tissue Proteins Nucleobindins Oncogene Proteins, Fusion (genetics) Promoter Regions, Genetic RUNX1 Translocation Partner 1 Protein Transcription Factors (genetics) Transcriptional Activation

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