Abstract | BACKGROUND: METHODS: Lethally irradiated female EPP mice were transplanted with bone marrow cells from healthy male mice and were monitored during 12 or 36 weeks. Two groups of animals killed 12 weeks after transplant were also treated with granulocyte colony-stimulating factor. RESULTS:
Cell transplantation decreased porphyrin contents in erythrocytes and liver. Improved hepatic structure and function and reduced hepatic fibrosis were observed, especially 36 weeks after transplant. Bone marrow-derived cells (22%-35%) were identified in the liver of recipient mice by means of fluorescence in situ hybridization (chrY-FISH) or green fluorescent protein staining and were characterized by immunofluorescence staining. The livers of recipients contained 20% to 30% myofibroblasts (alpha-smooth muscle actin-positive cells), 40% CK19-positive cells, and 10% to 28% hepatocytes ( albumin-positive cells) derived from the donor bone marrow. CONCLUSIONS: Bone marrow-derived cells play a significant role in restoring and regenerating hepatic tissue in EPP mice. Hepatic repair was associated with fibrogenesis, enhanced by granulocyte colony-stimulating factor treatment, and almost normal liver structure and function was observed in the long term (36 weeks posttransplant).
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Authors | María García-Bravo, María-Josefa Morán-Jiménez, Oscar Quintana-Bustamante, Manuel Méndez, Inmaculada Gutiérrez-Vera, Juan Bueren, Eduardo Salido, José-Carlos Segovia, Antonio Fontanellas, Rafael Enríquez de Salamanca |
Journal | Transplantation
(Transplantation)
Vol. 88
Issue 12
Pg. 1332-40
(Dec 27 2009)
ISSN: 1534-6080 [Electronic] United States |
PMID | 20029329
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Recombinant Proteins
- Granulocyte Colony-Stimulating Factor
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Topics |
- Animals
- Bone Marrow Cells
(cytology)
- Bone Marrow Transplantation
(methods)
- Disease Models, Animal
- Female
- Granulocyte Colony-Stimulating Factor
(therapeutic use)
- In Situ Hybridization, Fluorescence
- Liver
(pathology)
- Liver Regeneration
(physiology)
- Male
- Mice
- Mice, Inbred BALB C
- Protoporphyria, Erythropoietic
(drug therapy, pathology, surgery)
- Recombinant Proteins
- Treatment Outcome
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