Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity.

Abstract

Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1-->4)-linked beta-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC(50)) from 0.22 to 1.37mug/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry.

• Authors: Pinaki Mandal, Carlos Alberto Pujol, Elsa Beatriz Damonte, Tuhin Ghosh, Bimalendu Ray
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 46 Issue 2 Pg. 173-8 (Mar 01 2010) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 20026104 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2009 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• Glycosides
• Xylans
• Sulfur

Topics

• Animals
• Antiviral Agents (chemistry, isolation & purification, pharmacology)
• Chlorocebus aethiops
• Chromatography, Gel
• Glycosides (metabolism)
• Herpesvirus 1, Human (drug effects)
• Herpesvirus 2, Human (drug effects)
• Magnetic Resonance Spectroscopy
• Molecular Weight
• Rhodophyta (chemistry)
• Sulfur (metabolism)
• Vero Cells
• Xylans (chemistry, isolation & purification, pharmacology)