Analytical protocol for identification of BMAA and DAB in biological samples.

Abstract

Beta-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.

• Authors: Zdenek Spácil, Johan Eriksson, Sara Jonasson, Ulla Rasmussen, Leopold L Ilag, Birgitta Bergman
• Journal: The Analyst (Analyst) Vol. 135 Issue 1 Pg. 127-32 (Jan 2010) ISSN: 1364-5528 [Electronic] England
• PMID: 20024192 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids, Diamino
• Aminobutyrates
• Cyanobacteria Toxins
• beta-N-methylamino-L-alanine
• 2,3-diaminobutanoic acid

Topics

• Amino Acids, Diamino (analysis, isolation & purification)
• Aminobutyrates (analysis, isolation & purification)
• Chromatography, High Pressure Liquid (methods)
• Cyanobacteria (chemistry, metabolism)
• Cyanobacteria Toxins
• Humans
• Neurodegenerative Diseases (metabolism)
• Solid Phase Extraction
• Spectrometry, Mass, Electrospray Ionization (methods)