Using BCG, MPT-51 and Ag85 as antigens in an indirect ELISA for the diagnosis of bovine tuberculosis.

Abstract

This study evaluated the Mycobacterium tuberculosis protein antigen MPT-51, the trimeric antigen 85 (Ag85) complex, and Bacillus Calmette-Guérin (BCG) in an indirect ELISA to diagnose bovine tuberculosis (TB) from serum samples. Serum was collected from 208 intra-dermal tuberculin test (ITT)-positive and 54 ITT-negative animals from a region where bovine TB is endemic. Using the Ag85 and BCG antigens, the indirect ELISA was able to discriminate ITT-positive from ITT-negative animals. This level of discrimination was not achieved when using the MPT-51 antigen. The highest sensitivity (Se) and specificity (Sp) of the test was found when BCG was used (Se, 82%; Sp, 91%). Further work in different epidemiological settings and with larger numbers of animals will be required to validate these findings.

• Authors: Ediane Batista da Silva, Bruna Daniella de Souza Silva, Jerônimo Rafael Ruiz Leon, André Kipnis, Isabel K F de Miranda Santos, Ana Paula Junqueira-Kipnis
• Journal: Veterinary journal (London, England : 1997) (Vet J) Vol. 187 Issue 2 Pg. 276-8 (Feb 2011) ISSN: 1532-2971 [Electronic] England
• PMID: 20022773 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2009 Elsevier Ltd. All rights reserved.

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• MPT51 protein, Mycobacterium
• antigen 85, Mycobacterium bovis
• Mycobacterium tuberculosis antigens

Topics

• Animals
• Antigens, Bacterial (blood, immunology)
• Bacterial Proteins (blood, immunology)
• Cattle
• Enzyme-Linked Immunosorbent Assay (methods, veterinary)
• Mycobacterium bovis (immunology)
• Sensitivity and Specificity
• Tuberculin Test (veterinary)
• Tuberculosis, Bovine (blood, diagnosis)