[Simulation of corneal epithelial injuries by mechanical and corrosive damage : Influence of fetal bovine serum and dexpanthenol on epithelial regeneration in a cell culture model].

Abstract

INTRODUCTION:

The present study describes simulation of corneal epithelial injury and its regeneration using an in-vitro model of immortalized human corneal epithelial cells (HCE-T) growing as monolayer cultures.

MATERIAL AND METHODS:

The epithelial model was damaged using defined strengths by mechanical injury or partial damage using chemical detergents (SDS and acidified medium) and subsequently the epithelium was further cultivated using serum-containing and serum-free medium supplemented with varying concentrations of calcium pantothenat. After mechanical injury wound healing was evaluated using a photomicroscope over a period of up to 48 h whereas after chemical injury a cell viability assay was used to detect the course of ATP levels in the cell layers as an indicator for the metabolic activity.

RESULTS:

Depending on the kind of injury pantothenat showed a regeneration enhancing effect in the concentration range from 0.001% to 0.01%. However, a concentration of 0.1% pantothenat appeared to be regeneration inhibiting. The combination of pantothenat and serum was more beneficial for wound healing than pantothenat alone, whereas serum partly levelled the effect of pantothenat.

CONCLUSION:

The described model allowed simulation of corneal epithelial injury and its regeneration, whereby the influence of the serum content and the kind of injury could be determined.

• Authors: M Hahne, S Reichl
• Journal: Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft (Ophthalmologe) Vol. 107 Issue 6 Pg. 529-32, 534-6 (Jun 2010) ISSN: 1433-0423 [Electronic] Germany
• Vernacular Title: Simulation von Korneaepithelverletzungen mittels mechanischer und korrosiver Schädigung : Beeinflussung der Regeneration durch fötales Kälberserum und Dexpanthenol am Zellkulturmodell.
• PMID: 20020304 (Publication Type: Journal Article)

Chemical References

• Surface-Active Agents
• Pantothenic Acid
• dexpanthenol
• Serum Albumin, Bovine
• Sodium Dodecyl Sulfate

Topics

• Animals
• Burns, Chemical (pathology)
• Cattle
• Cell Line
• Cell Survival (drug effects)
• Epithelial Cells (drug effects)
• Epithelium, Corneal (drug effects, injuries, pathology)
• Eye Burns (pathology)
• Female
• Humans
• Hydrogen-Ion Concentration
• In Vitro Techniques
• Middle Aged
• Pantothenic Acid (analogs & derivatives, pharmacology)
• Regeneration (drug effects)
• Serum Albumin, Bovine (pharmacology)
• Sodium Dodecyl Sulfate (toxicity)
• Surface-Active Agents (toxicity)