[Effects of thapsigargicin on Ca2+ movements in L1210 cells permeabilized with digitonin].

Abstract

The effect of Thapsigargicin (TGC), a non-phorbol ester type tumor promoter, on Ca2+ movements has been investigated using L1210 mouse lymphoma cells. Ca2+ release from intact and digitonin permeabilized cells was evaluated using Fura-2 and Fura-3. TGC like Thapsigargin (TG) has the ability to discharge the intracellular Ca2+ stores and to increase intracellular free Ca2+ concentrations. TGC in a concentration dependent manner (0.16-16 nM) also inhibited cell growth and this effect was at least partially reversed by arachidonate.

• Authors: E Oztetik
• Journal: Biomeditsinskaia khimiia (Biomed Khim) 2009 Sep-Oct Vol. 55 Issue 5 Pg. 651-62 ISSN: 2310-6972 [Print] Russia (Federation)
• PMID: 20017396 (Publication Type: English Abstract, Journal Article)

Chemical References

• Lactones
• Sesquiterpenes
• thapsigargicin
• Digitonin
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Cell Line, Tumor
• Cell Membrane Permeability (drug effects)
• Digitonin (chemistry)
• Lactones (pharmacology)
• Leukemia L1210 (metabolism)
• Mice
• Sesquiterpenes (pharmacology)