Abstract | PURPOSE: MATERIALS AND METHODS: One hundred twenty-two HNCA patients, 42 NPC, 66 laryngeal carcinoma, and 14 hypopharyngeal carcinoma and 3 groups of 100 control subjects were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to find a specific cutoff value for the NPC-related seropositivity of EBV IgG antibodies. RESULTS: NPC group showed higher serum level of EBV IgG antibodies than control and other HNCA groups (P < .05). However, the traditional cutoff value, mean + 2 SDs of control subjects, failed to demarcate the seropositives of NPC patients from those of healthy population (P > .05). The new cutoff value, mean + 2 SDs of the seropositives group of control subjects who had already been grouped by the traditional cutoff value, proved successful. It succeeded to demarcate between the NPC-related EBV IgG seropositivity and that issued from the persistent, latent, or reactivated EBV infection in the population (P < .05). The sensitivity/specificity of NPC detection by the new cutoff-based ELISA kit, 76.19% and 86%, was close or higher than that of EBV IgA antibodies. CONCLUSION: EBV IgG-based ELISA could be used for the diagnosis of NPC using a new cutoff threshold that excludes the population baseline of EBV IgG seropositivity.
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Authors | Ahmed S Abdulamir, Rand R Hafidh, Fatimah Abu Bakar, Kassim Abbas |
Journal | American journal of otolaryngology
(Am J Otolaryngol)
2010 Nov-Dec
Vol. 31
Issue 6
Pg. 410-7
ISSN: 1532-818X [Electronic] United States |
PMID | 20015794
(Publication Type: Journal Article)
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Copyright | Copyright © 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Antibodies
- Immunoglobulin G
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Topics |
- Adolescent
- Adult
- Aged
- Antibodies
(analysis)
- Carcinoma
- Enzyme-Linked Immunosorbent Assay
- Epstein-Barr Virus Infections
(immunology)
- Female
- Head and Neck Neoplasms
(immunology)
- Herpesvirus 4, Human
(immunology)
- Humans
- Hypopharyngeal Neoplasms
(immunology)
- Immunoglobulin G
(immunology)
- Laryngeal Neoplasms
(immunology)
- Male
- Middle Aged
- Nasopharyngeal Carcinoma
- Nasopharyngeal Neoplasms
(diagnosis, immunology)
- Sensitivity and Specificity
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