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Novel Epstein-Barr virus immunoglobulin G-based approach for the specific detection of nasopharyngeal carcinoma.

AbstractPURPOSE:
This study was designed to find a reliable Epstein-Barr virus (EBV) immunoglobulin (Ig) G-based diagnostic/screening test for nasopharyngeal carcinoma (NPC) able to demarcate between the NPC-related seropositivity of EBV IgG antibodies and that of other head and neck cancer (HNCA) and control groups. The NPC-associated immunosuppression affects EBV IgA much more than IgG, leading to inconsistent detection of NPC using EBV IgA antibodies.
MATERIALS AND METHODS:
One hundred twenty-two HNCA patients, 42 NPC, 66 laryngeal carcinoma, and 14 hypopharyngeal carcinoma and 3 groups of 100 control subjects were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to find a specific cutoff value for the NPC-related seropositivity of EBV IgG antibodies.
RESULTS:
NPC group showed higher serum level of EBV IgG antibodies than control and other HNCA groups (P < .05). However, the traditional cutoff value, mean + 2 SDs of control subjects, failed to demarcate the seropositives of NPC patients from those of healthy population (P > .05). The new cutoff value, mean + 2 SDs of the seropositives group of control subjects who had already been grouped by the traditional cutoff value, proved successful. It succeeded to demarcate between the NPC-related EBV IgG seropositivity and that issued from the persistent, latent, or reactivated EBV infection in the population (P < .05). The sensitivity/specificity of NPC detection by the new cutoff-based ELISA kit, 76.19% and 86%, was close or higher than that of EBV IgA antibodies.
CONCLUSION:
EBV IgG-based ELISA could be used for the diagnosis of NPC using a new cutoff threshold that excludes the population baseline of EBV IgG seropositivity.
AuthorsAhmed S Abdulamir, Rand R Hafidh, Fatimah Abu Bakar, Kassim Abbas
JournalAmerican journal of otolaryngology (Am J Otolaryngol) 2010 Nov-Dec Vol. 31 Issue 6 Pg. 410-7 ISSN: 1532-818X [Electronic] United States
PMID20015794 (Publication Type: Journal Article)
CopyrightCopyright © 2010 Elsevier Inc. All rights reserved.
Chemical References
  • Antibodies
  • Immunoglobulin G
Topics
  • Adolescent
  • Adult
  • Aged
  • Antibodies (analysis)
  • Carcinoma
  • Enzyme-Linked Immunosorbent Assay
  • Epstein-Barr Virus Infections (immunology)
  • Female
  • Head and Neck Neoplasms (immunology)
  • Herpesvirus 4, Human (immunology)
  • Humans
  • Hypopharyngeal Neoplasms (immunology)
  • Immunoglobulin G (immunology)
  • Laryngeal Neoplasms (immunology)
  • Male
  • Middle Aged
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms (diagnosis, immunology)
  • Sensitivity and Specificity

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