Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: MATERIALS AND METHODS: By semi-quantitative/quantitative reverse transcription-PCR, we investigated the transcript level of STS in MCF-7 cells treated with various concentrations of SGLXD. By using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells and dual luciferase reporter (DLR) based bioluminescent measurements, we evaluated the enzymatic activity of STS after SGLXD treatment. RESULTS: By RT-PCR and real time PCR, the mRNA level of STS was decreased by SGLXD treatment, in the dose-dependent manner, compared to negative control (p<0.01). By DLR assay, different concentrations of SGLXD significantly inhibited the enzymatic activity of STS in MCF-7 cells dose-dependently (p<0.05). CONCLUSIONS:
SGLXD could act as a selective estrogen enzyme modulator by down-regulating the STS expression in MCF-7 cells.
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Authors | Yi Zhang, Ping-Ping Li |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 127
Issue 3
Pg. 620-4
(Feb 17 2010)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 20015473
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- Estrogens
- RNA, Messenger
- Luciferases
- Steryl-Sulfatase
|
Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Breast Neoplasms
(drug therapy, metabolism)
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Down-Regulation
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Estrogens
(biosynthesis)
- Female
- Gene Expression Regulation, Enzymologic
(drug effects)
- Humans
- Luciferases
- Plants, Medicinal
- Plasmids
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Steryl-Sulfatase
(genetics, metabolism)
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