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Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells.

AbstractETHNOPHARMACOLOGICAL RELEVANCE:
Shu-Gan-Liang-Xue Decoction (SGLXD), a traditional Chinese herbal formula, has been used to ameliorate hot flushes symptom in breast cancer patients for decades.
AIM OF THE STUDY:
Steroid sulfatase (STS) has a crucial role in regulating the estrogen biosynthesis within breast tumors. We aimed to investigate whether SGLXD could modulate STS expression in human breast carcinoma MCF-7 cells.
MATERIALS AND METHODS:
By semi-quantitative/quantitative reverse transcription-PCR, we investigated the transcript level of STS in MCF-7 cells treated with various concentrations of SGLXD. By using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells and dual luciferase reporter (DLR) based bioluminescent measurements, we evaluated the enzymatic activity of STS after SGLXD treatment.
RESULTS:
By RT-PCR and real time PCR, the mRNA level of STS was decreased by SGLXD treatment, in the dose-dependent manner, compared to negative control (p<0.01). By DLR assay, different concentrations of SGLXD significantly inhibited the enzymatic activity of STS in MCF-7 cells dose-dependently (p<0.05).
CONCLUSIONS:
SGLXD could act as a selective estrogen enzyme modulator by down-regulating the STS expression in MCF-7 cells.
AuthorsYi Zhang, Ping-Ping Li
JournalJournal of ethnopharmacology (J Ethnopharmacol) Vol. 127 Issue 3 Pg. 620-4 (Feb 17 2010) ISSN: 1872-7573 [Electronic] Ireland
PMID20015473 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2009 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Drugs, Chinese Herbal
  • Estrogens
  • RNA, Messenger
  • Luciferases
  • Steryl-Sulfatase
Topics
  • Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
  • Breast Neoplasms (drug therapy, metabolism)
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • Down-Regulation
  • Drugs, Chinese Herbal (pharmacology, therapeutic use)
  • Estrogens (biosynthesis)
  • Female
  • Gene Expression Regulation, Enzymologic (drug effects)
  • Humans
  • Luciferases
  • Plants, Medicinal
  • Plasmids
  • RNA, Messenger (metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Steryl-Sulfatase (genetics, metabolism)

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