Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection.

Abstract

Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3'-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.

• Authors: Yasuyuki Yamaji, Keitaro Sakurai, Koji Hamada, Ken Komatsu, Johji Ozeki, Akiko Yoshida, Atsushi Yoshii, Takumi Shimizu, Shigetou Namba, Tadaaki Hibi
• Journal: Archives of virology (Arch Virol) Vol. 155 Issue 2 Pg. 263-8 (Feb 2010) ISSN: 1432-8798 [Electronic] Austria
• PMID: 20012112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Elongation Factor 1

Topics

• Gene Knockdown Techniques
• Gene Silencing
• Genetic Vectors
• Host-Pathogen Interactions
• Peptide Elongation Factor 1 (metabolism)
• Plant Diseases (virology)
• Potexvirus (genetics)
• Protein Biosynthesis
• Tobacco (virology)
• Tobacco Mosaic Virus (pathogenicity)