Extracellular SOD and VEGF are increased in vitreous bodies from proliferative diabetic retinopathy patients.

Abstract

PURPOSE:

To evaluate the relationship between vascular endothelial growth factor (VEGF) and extracellular superoxide dismutase (EC-SOD) in vitreous body and serum in patients with proliferative diabetic retinopathy (PDR), and investigate the role of EC-SOD in PDR by evaluating its angiostatic effect, using an in vitro angiogenesis model. To investigate the role of EC-SOD in PDR by evaluating its angiostatic effect, using an in vitro angiogenesis model.

METHODS:

EC-SOD and VEGF concentrations in vitreous and serum samples from PDR and macular hole (MH) were measured by ELISA. The effects of EC-SOD on VEGF-induced proliferation, migration, and tube formation were evaluated using human umbilical vein endothelial cells (HUVECs). Moreover, the effects of EC-SOD on VEGF-induced proliferation and migration were evaluated in HUVECs and primary normal human retinal microvascular endothelial cells.

RESULTS:

Intravitreal concentrations of EC-SOD were significantly higher (p<0.01) in PDR (58.0+/-23.8 ng/ml, mean+/-SD) than in MH (29.3+/-6.6 ng/ml). Intravitreal concentrations of VEGF were dramatically higher (p<0.01) in PDR (798.2+/-882.7 pg/ml) than in MH (17.7+/-15.5 pg/ml). The serum concentrations of EC-SOD and VEGF did not differ between the two patient groups. The vitreous concentrations of VEGF correlated with those of EC-SOD in all patients (rs=0.61, p<0.001). In HUVECs, EC-SOD at 100 ng/ml significantly suppressed VEGF-induced proliferation and tube formation, but not VEGF-induced migration.

CONCLUSIONS:

EC-SOD was increased together with VEGF in the vitreous body from PDR patients, suggesting that EC-SOD may play a pivotal role in the pathogenesis of angiogenesis.

• Authors: Hiroshi Izuta, Yuichi Chikaraishi, Tetsuo Adachi, Masamitsu Shimazawa, Tetsuya Sugiyama, Tsunehiko Ikeda, Hideaki Hara
• Journal: Molecular vision (Mol Vis) Vol. 15 Pg. 2663-72 (Dec 10 2009) ISSN: 1090-0535 [Electronic] United States
• PMID: 20011081 (Publication Type: Journal Article)

Chemical References

• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Superoxide Dismutase

Topics

• Cell Movement
• Cell Proliferation
• Diabetic Retinopathy (blood, complications, enzymology, pathology)
• Endothelial Cells (enzymology, pathology)
• Extracellular Space (enzymology)
• Female
• Humans
• Male
• Middle Aged
• Neovascularization, Pathologic (complications, enzymology)
• Retinal Perforations (blood, complications, enzymology, pathology)
• Superoxide Dismutase (blood)
• Vascular Endothelial Growth Factor A (blood)
• Vitreous Body (metabolism, pathology)