Short periodic applications of the vacuum-assisted closure device cause an extended tissue response in the diabetic mouse model.

Abstract

BACKGROUND:

The vacuum-assisted closure device is a widely used mechanical modulator of wound healing; however, the optimal time kinetics of application have not been determined. The objective of the study was to optimize the kinetics of vacuum-assisted closure application.

METHODS:

Full-thickness wounds in seven diabetic mice per study group were treated with either an occlusive dressing alone, the vacuum-assisted closure device for 6 or 12 hours, or the vacuum-assisted closure device periodically for 4 hours every other day or continuously for 7 days. Wound closure and tissue response were evaluated by macroscopic, histologic, and immunohistochemical analyses on day 7.

RESULTS:

Wound closure was significantly faster after short initial vacuum-assisted closure (6-hour and 12-hour groups) when compared with continuous treatment. Increased granulation tissue formation was seen in the 12-hour group (2.4-fold increase) and in those treated periodically for 4 hours every other day (3.2-fold increase) compared with the dressing-alone controls. Significant stimulation of cell proliferation was seen after all vacuum-assisted closure patterns (3.6- to 5.3-fold increase), whereas angiogenesis was augmented only after the device was applied for either three times for 4 hours (4.3-fold) or continuously (4.7-fold) when compared with dressing-treated wounds. Treatment three times for 4 hours showed a superior angiogenic effect also when compared with short initial applications (6-hour and 12-hour groups).

CONCLUSIONS:

Short vacuum-assisted closure treatment induced an extended biological response in the wound. A total of 12 hours of periodically applied vacuum-assisted closure reached a similar wound tissue response as continuously applied vacuum-assisted closure for 7 days. These findings suggest new clinical approaches for mechanical wound-healing devices.

• Authors: Sandra Saja Scherer, Giorgio Pietramaggiori, Jasmine C Mathews, Dennis P Orgill
• Journal: Plastic and reconstructive surgery (Plast Reconstr Surg) Vol. 124 Issue 5 Pg. 1458-1465 (Nov 2009) ISSN: 1529-4242 [Electronic] United States
• PMID: 20009831 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Biomarkers
• Ki-67 Antigen
• Vascular Cell Adhesion Molecule-1

Topics

• Animals
• Biomarkers (metabolism)
• Cell Proliferation
• Diabetes Mellitus, Experimental
• Granulation Tissue (metabolism)
• Immunohistochemistry
• Ki-67 Antigen (metabolism)
• Kinetics
• Mice
• Negative-Pressure Wound Therapy (instrumentation)
• Neovascularization, Physiologic
• Occlusive Dressings
• Time Factors
• Vascular Cell Adhesion Molecule-1 (metabolism)
• Wound Healing (physiology)