Abstract |
This study used an in vivo mouse model to analyze the response of dendritic cells (DCs) in Peyer's patches (PPs) within the first 48 h of infection with the wild-type murine rotavirus EDIM ( EDIM(wt)). After the infection, the absolute number of DCs was increased by 2-fold in the PPs without a modification of their relative percentage of the total cell number. Also, the DCs from PPs of infected mice showed a time-dependent migration to the subepithelial dome (SED) and an increase of the surface activation markers CD40, CD80, and CD86. This response was more evident at 48 h postinfection (p.i.) and depended on viral replication, since DCs from PPs of mice inoculated with UV-treated virus did not show this phenotype. As a result of the activation, the DCs showed an increase in the expression of mRNA for the proinflammatory cytokines interleukin-12/23p40 ( IL-12/23p40), tumor necrosis factor alpha ( TNF-alpha), and beta interferon (IFN-beta), as well as for the regulatory cytokine IL-10. These results suggest that, a short time after rotavirus infection, the DCs from PPs play a critical role in controlling the infection and, at the same time, avoiding an excessive inflammatory immune response.
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Authors | Delia V Lopez-Guerrero, Selene Meza-Perez, Oscar Ramirez-Pliego, Maria A Santana-Calderon, Pavel Espino-Solis, Lourdes Gutierrez-Xicotencatl, Leopoldo Flores-Romo, Fernando R Esquivel-Guadarrama |
Journal | Journal of virology
(J Virol)
Vol. 84
Issue 4
Pg. 1856-66
(Feb 2010)
ISSN: 1098-5514 [Electronic] United States |
PMID | 20007263
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- B7-1 Antigen
- B7-2 Antigen
- CD40 Antigens
- Cd86 protein, mouse
- Cytokines
- DNA Primers
- Inflammation Mediators
- RNA, Messenger
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Topics |
- Animals
- B7-1 Antigen
(metabolism)
- B7-2 Antigen
(metabolism)
- Base Sequence
- CD40 Antigens
(metabolism)
- Cell Movement
(immunology)
- Cytokines
(genetics)
- DNA Primers
(genetics)
- Dendritic Cells
(immunology, pathology)
- Disease Models, Animal
- Female
- Inflammation Mediators
(metabolism)
- Mice
- Mice, Inbred BALB C
- Peyer's Patches
(immunology, pathology)
- RNA, Messenger
(genetics, metabolism)
- Rotavirus Infections
(immunology, pathology, virology)
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