Seven consecutive successful clinical islet isolations with pancreatic ductal injection.

Abstract

Inconsistent islet isolation is one of the issues of clinical islet transplantation. In the current study, we applied ductal injection to improve the consistency of islet isolation. Seven islet isolations were performed with the ductal injection of ET-Kyoto solution (DI group) and eight islet isolations were performed without the ductal injection (standard group) using brain-dead donor pancreata. Isolated islets were evaluated based on the Edmonton protocol for transplantation. The DI group had significantly higher islet yields (588,566 +/- 64,319 vs. 354,836 +/- 89,649 IE, p < 0.01) and viability (97.3 +/- 1.2% vs. 92.6 +/- 1.2%, p < 0.02) compared with the standard group. All seven isolated islet preparations in the DI group (100%), versus only three out of eight isolated islet preparations (38%) in the standard group met transplantation criteria. The islets from the DI group were transplanted into three type 1 diabetic patients and all three patients became insulin independent. Ductal injection significantly improved quantity and quality of isolated islets and resulted in high success rate of clinical islet transplantation. This simple modification will reduce the risk of failure of clinical islet isolation.

• Authors: Shinichi Matsumoto, Hirofumi Noguichi, Masayuki Shimoda, Tetsuya Ikemoto, Bashoo Naziruddin, Andrew Jackson, Yoshiko Tamura, Greg Olson, Yasutaka Fujita, Daisuke Chujo, Morihito Takita, Naoya Kobayashi, Nicholas Onaca, Marlon Levy
• Journal: Cell transplantation (Cell Transplant) Vol. 19 Issue 3 Pg. 291-7 ( 2010) ISSN: 1555-3892 [Electronic] United States
• PMID: 19995483 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adult
• Cell Separation (methods, standards)
• Diabetes Mellitus, Type 1 (surgery)
• Dissection (methods, standards)
• Female
• Humans
• Injections (methods)
• Islets of Langerhans (cytology)
• Islets of Langerhans Transplantation (methods, standards)
• Male
• Tissue and Organ Harvesting (methods, standards)
• Treatment Outcome