Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.

Abstract

Stimulation of M3 muscarinic receptors expressed by SH-SY5Y cells induced a dose- and time-related increase in the mass of Ins(1,4,5)P3 (basal 38.3 +/- 5.8 pmol/mg of protein) and Ins(1,3,4,5)P4 (basal 6.1 +/- 1.2 pmol/mg of protein). Comparison of radioreceptor mass assays with [3H]inositol labelling showed higher-fold stimulations with the former protocol. The later accumulation of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass was dependent upon extracellular Ca2+.

• Authors: D G Lambert, R A Challiss, S R Nahorski
• Journal: The Biochemical journal (Biochem J) Vol. 273 ( Pt 3) Pg. 791-4 (Feb 01 1991) ISSN: 0264-6021 [Print] England
• PMID: 1996973 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inositol Phosphates
• Receptors, Muscarinic
• inositol-1,3,4,5-tetrakisphosphate
• Inositol
• Inositol 1,4,5-Trisphosphate
• Carbachol

Topics

• Animals
• Carbachol (pharmacology)
• Cell Line
• Inositol (metabolism)
• Inositol 1,4,5-Trisphosphate (metabolism)
• Inositol Phosphates (isolation & purification, metabolism)
• Kinetics
• Mice
• Neuroblastoma
• Receptors, Muscarinic (drug effects, physiology)