Abstract | AIM OF THE STUDY: MATERIALS AND METHODS: The structure of GSPP was characterized by high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, beta-elimination reaction, and NMR spectroscopy. SMMC-7721 cells were used to assess the influence of GSPP on hepatocellular carcinoma. Cell proliferation and survival was determined by trypan blue exclusion assay. Cell migration was performed by wound-healing and transwell assay. The secretion of IL-8 was detected by an enzyme-linked immunosorbent assay kit. Flow cytometry was used to analyze intracellular calcium concentration, as well as cell cycle distribution and apoptosis. Confocal microscopy was used to assess the localization and configuration of actin filaments. RESULTS: GSPP was chemically characterized as a sulfated polysaccharide- protein complex with O- glycopeptide linkages. Our results showed that GSPP inhibited the proliferation of SMMC-7721 cells and blocked cells in the S phase. No direct toxicity against cells was observed. Furthermore, GSPP inhibited the migration of SMMC-7721 cells with the reduction of intracellular calcium. Actin filaments were polymerized and accumulated in the cytoplasm of the treated cells, whereas the secretion of IL-8 was not significantly changed after GSPP exposure. CONCLUSION:
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Authors | Dan Chen, Wei-Juan Yao, Xiao-Lan Zhang, Xiao-Qiang Han, Xiao-Yuan Qu, Wei-Bo Ka, Da-Gong Sun, Xiong-Zhi Wu, Zong-Yao Wen |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 127
Issue 3
Pg. 702-8
(Feb 17 2010)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 19969061
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2009 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Actins
- Antineoplastic Agents
- Glycoproteins
- Interleukin-8
- Polysaccharides
- gepsin
- Calcium
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Topics |
- Actin Cytoskeleton
(metabolism)
- Actins
(metabolism)
- Animals
- Antineoplastic Agents
(chemistry, isolation & purification, pharmacology, therapeutic use)
- Calcium
(metabolism)
- Carcinoma, Hepatocellular
(drug therapy, secondary)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Glycoproteins
(chemistry, pharmacology, therapeutic use)
- Interleukin-8
(metabolism)
- Liver Neoplasms
(drug therapy, metabolism, secondary)
- Lizards
- Medicine, Chinese Traditional
- Neoplasm Invasiveness
- Organotherapy
- Polysaccharides
(pharmacology, therapeutic use)
- S Phase
(drug effects)
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