[Mechanism of endothelial Rho/Rho kinase in extravascular migration of fibrosarcoma cell].

Abstract

OBJECTIVE:

To study the mechanism of endothelial Rho/Rho kinase in extravascular migration of fibrosarcoma cell.

METHODS:

We used an in vitro model of fibrosarcoma cell transmigration across a monolayer of human umbilical vein endothelial cell (HUVEC) cultured on collagen gel to observe extravascular migration of fibrosarcoma cells, and then calculated the electrical resistance of HUVEC monolayer and endothelial myosin light chain (MLC) phosphorylation in extravascular migration of fibrosarcoma cells.

RESULTS:

Fibrosarcoma cells migrated through endothelial cells into collagen gel. The electrical resistance of a HUVEC monolayer reduced and endothelial MLC phosphorylation enhanced in the extravascular migration of fibrosarcoma cells. Endothelial Rho inhibitor (C3 transferase) and Rho kinase inhibitor (Y-27632) inhibited the extravascular migration of fibrosarcoma cells and inhibited the reduction of electrical resistance of a HUVEC monolayer and the enhancement of endothelial MLC phosphorylation in extravascular migration of fibrosarcoma cells.

CONCLUSION:

Endothelial Rho/Rho kinase may regulate fibrosarcoma cell transendothelial migration through MLC phosphorylation.

• Authors: Hua Xin, Zhong-Li Gao, Zhen-Guo Han
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 31 Issue 5 Pg. 594-7 (Oct 2009) ISSN: 1000-503X [Print] China
• PMID: 19968078 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amides
• Enzyme Inhibitors
• Pyridines
• Y 27632
• rho-Associated Kinases
• rho GTP-Binding Proteins

Topics

• Amides (pharmacology)
• Cell Movement (physiology)
• Cells, Cultured
• Enzyme Inhibitors (pharmacology)
• Fibrosarcoma (pathology)
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Phosphorylation
• Pyridines (pharmacology)
• rho GTP-Binding Proteins (metabolism, physiology)
• rho-Associated Kinases (metabolism, physiology)