Abstract | AIM: METHODS: A standard hybridoma method was used to generate monoclonoal antibodies against recombinant human S100A4. ELISA, Western blot and immunohistochemistry were used to validate this antibody and the antibody is used to examine specimens of human breast and colon cancers. RESULTS: CONCLUSION: The monoclonal antibody is specific for S100A4 and can be produced on a large scale; therefore it will be more reproducible for future clinical applications.
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Authors | Xiang-Yang Hu, Xiao-Ming Huang, Nan Zhang, Dao-Wei He, Yin-Ying Ye |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 25
Issue 12
Pg. 1136-9
(Dec 2009)
ISSN: 1007-8738 [Print] China |
PMID | 19961801
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Antibodies, Monoclonal
(immunology)
- Antibody Specificity
- Enzyme-Linked Immunosorbent Assay
- Humans
- Hybridomas
- Immunohistochemistry
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