Abstract | OBJECTIVES: METHODS: We analyzed hTERT expression, telomerase activity and invasion capacity in prostate cancer cell lines overexpressing the wild-type or mutant form of FADD (S194D or A). FADD, phosphorylated FADD (p-FADD) and hTERT expression in viable prostate cancer cells following NHT were immunohistochemically examined using 50 prostatectomy samples. RESULTS: Dephosphorylated FADD (S194A) overexpression enhanced hTERT expression and telomerase activity, resulting in increased cell proliferation and invasion capacity. In Kaplan-Meier survival analysis, the patients with prostate cancer expressing low levels of p-FADD and high levels of hTERT had significantly higher rates of biochemical recurrence than those with high p-FADD and low hTERT expression (p < 0.001). CONCLUSIONS: The phosphorylation status of FADD at serine 194 could strongly affect survival and invasion of prostate cancer cells via modulation of hTERT expression and telomerase activity. p-FADD and hTERT expression may have potential as new biomarkers predicting the biochemical recurrence after NHT.
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Authors | Yoshiaki Matsumura, Keiji Shimada, Nobumichi Tanaka, Kiyohide Fujimoto, Yoshihiko Hirao, Noboru Konishi |
Journal | Pathobiology : journal of immunopathology, molecular and cellular biology
(Pathobiology)
Vol. 76
Issue 6
Pg. 293-302
( 2009)
ISSN: 1423-0291 [Electronic] Switzerland |
PMID | 19955841
(Publication Type: Journal Article)
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Copyright | Copyright 2009 S. Karger AG, Basel. |
Chemical References |
- Androgen Antagonists
- Biomarkers, Tumor
- Fas-Associated Death Domain Protein
- TERT protein, human
- Telomerase
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Topics |
- Adenocarcinoma
(metabolism, pathology, therapy)
- Aged
- Androgen Antagonists
(therapeutic use)
- Animals
- Apoptosis
- Biomarkers, Tumor
(metabolism)
- Cell Line, Tumor
- Cell Survival
(physiology)
- Chick Embryo
- Chorioallantoic Membrane
(blood supply)
- Disease Progression
- Drug Therapy, Combination
- Fas-Associated Death Domain Protein
(metabolism)
- Humans
- Male
- Neoadjuvant Therapy
- Neoplasm Invasiveness
- Phosphorylation
- Prostatectomy
- Prostatic Neoplasms
(metabolism, pathology, therapy)
- Telomerase
(metabolism)
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