Abstract | OBJECTIVE: METHODS: Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed. RESULTS: The metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%. CONCLUSION:
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Authors | Qiong Liu, Wei Xu, Hai-rong Qiu, Rong Wang, Hui Yu, Lei Fan, Kou-rong Miao, Jian-yong Li |
Journal | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
(Zhonghua Xue Ye Xue Za Zhi)
Vol. 30
Issue 9
Pg. 601-4
(Sep 2009)
ISSN: 0253-2727 [Print] China |
PMID | 19954619
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CpG-ODN DSP30
- Oligonucleotides
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Chromosome Aberrations
- Chromosome Banding
- Female
- Humans
- Karyotyping
- Leukemia, Lymphocytic, Chronic, B-Cell
(genetics, pathology)
- Male
- Middle Aged
- Oligonucleotides
(pharmacology)
- Tumor Cells, Cultured
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