Expression of CD133, PAX2, ESA, and GPR30 in invasive ductal breast carcinomas.

Abstract

BACKGROUND:

Biomarkers in breast neoplasms provide invaluable information regarding prognosis and help determining the optimal treatment. We have examined the possible correlation between cancer stem cell (CSC)-like markers (CD133, paired box gene 2 protein (PAX2), epithelial specific antigen (ESA)), and a new membrane estrogen receptor (G-protein coupled receptor 30 (GPR30)) in invasive ductal breast carcinomas with known clinicopathological parameters, tumor recurrence, and expression of some known biomarkers.

METHODS:

In 74 invasive ductal breast carcinomas, we investigated the protein expression of these molecular markers by immunohistochemistry, and their associations with known clinicopathological parameters, tumor recurrence, and expression of some known biomarkers. We studied the interrelationship between the expressions of these proteins.

RESULTS:

CD133, a putative CSC marker, was positively related to tumor size, tumor stage, and lymph node metastasis. PAX2 was negatively correlated with tumor recurrence. ESA, one of the breast CSC markers, was an indicator of tumor recurrence. GPR30 was associated with hormone receptors. Despite the correlation between GPR30 and the nuclear estrogen receptor, the expression was dependent. Positive staining of GPR30 in tumors displayed a significant association with high C-erbB2 expression and a tendency for tumor recurrence. A positive relationship between GPR30 and CD133 existed.

CONCLUSION:

Detecting the expression of CD133, PAX2, ESA, and GPR30 in invasive ductal breast carcinomas may be of help in more accurately predicting the aggressive properties of breast cancer and determining the optimal treatment.

• Authors: Qun Liu, Ji-guang Li, Xin-yu Zheng, Feng Jin, Hui-ting Dong
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 122 Issue 22 Pg. 2763-9 (Nov 20 2009) ISSN: 2542-5641 [Electronic] China
• PMID: 19951611 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AC133 Antigen
• Antigens, CD
• Biomarkers, Tumor
• GPER1 protein, human
• Glycoproteins
• Membrane Proteins
• PAX2 Transcription Factor
• PAX2 protein, human
• PROM1 protein, human
• Peptides
• Receptors, Estrogen
• Receptors, G-Protein-Coupled
• Receptors, Progesterone
• flotillins

Topics

• AC133 Antigen
• Adult
• Aged
• Antigens, CD (analysis)
• Biomarkers, Tumor (analysis)
• Breast Neoplasms (chemistry, pathology)
• Carcinoma, Ductal, Breast
• Female
• Glycoproteins (analysis)
• Humans
• Immunohistochemistry
• Membrane Proteins (analysis)
• Middle Aged
• Neoplasm Invasiveness
• Neoplastic Stem Cells (chemistry)
• PAX2 Transcription Factor (analysis)
• Peptides (analysis)
• Receptors, Estrogen (analysis)
• Receptors, G-Protein-Coupled (analysis)
• Receptors, Progesterone (analysis)