[Chromosome 13q deletion detected by interphase FISH in multiple myeloma: a study of 100 cases in China].

Abstract

OBJECTIVE:

To summarize the clinical significance and 13q- characters of 100 multiple myeloma (MM) patients detected by interphase fluorescence in situ hybridization (i-FISH).

METHODS:

Specimens of bone marrow were collected from 100 patients with MM who visited the Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences in Tianjin, China. Chromosome R-banding analysis and i-FISH were conducted.

RESULTS:

(1) i-FISH was used to investigate 100 patients with MM, whose median age was 56 years and 19 (19.0%) cases showed chromosome 13q deletions/monosony13 (Delta13); conventional cytogenetics (CC) revealed informative MM karyotypes in 24 patients (24.0%),with Delta13 in 10 (10.0%) of them. Detection rate of the two methods had no significant difference (P = 0.053), but in newly diagnosed patients i-FISH was much more sensitive than CC test (Detection rate, 25.3% vs 9.3%, P = 0.008). (2) Among the whole cases, 93 of them had complete follow-up information. The overall survival (OS) of the 93 patients was 41 (1-69) months. Univariate analysis showed that the positive rate of Delta13 detected by i-FISH >50%, clonal chromosome 13 abnormality (C13A), nonhyperdiploidy, Delta13 detected by both CC and i-FISH, beta2-MG > or = 3.5 mg/L were associated with significantly shorter OS. Multivariate analysis indicated that the positive rate of Delta13 detected by i-FISH > 50% and beta2-MG > or = 3.5 mg/L were the independent unfavorable factors. (3) According to the two independent unfavorable factors mentioned above, we divided the 93 patients into three groups: low-risk, standard-risk and high-risk. And there were significant differences of OS among the 3 groups (P < 0.05).

CONCLUSION:

(1) FISH studies demonstrate a high sensitivity at detecting chromosome 13 abnormality and should be used in the routine evaluation of MM. (2) The positive rate of Delta13 detected by i-FISH > 50% and beta2-MG > or = 3.5 mg/L were the independent adverse prognostic factors.

• Authors: Qian Li, Yun Lu, Ya-Fei Wang, Xu-Ping Liu, Jun-Yuan Qi, De-Hui Zou, Yao-Zhong Zhao, Lu-Gui Qiu
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 89 Issue 28 Pg. 1979-82 (Jul 28 2009) ISSN: 0376-2491 [Print] China
• PMID: 19950573 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adult
• Aged
• Chromosome Deletion
• Chromosomes, Human, Pair 13
• Female
• Humans
• In Situ Hybridization, Fluorescence (methods)
• Male
• Middle Aged
• Multiple Myeloma (diagnosis, genetics)
• Prognosis