Neolignans from plants in northeastern Brazil (Lauraceae) with activity against Trypanosoma cruzi.

Abstract

Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote growth was inhibited in 45% with licarin A and 20% with burchellin with an IC(50)/96 h of 462.7 microM and 756 microM, respectively. Epimastigotes treated with licarin A presented swollen mitochondria and disorganized mitochondrial cristae, kDNA and Golgi complex. When treated with burchellin, they presented enormous autophagosomes and chromatin disorganization. Licarin A and burchellin were able to induce trypomastigote death with IC(50)/24 h of 960 microM and 520 microM, respectively. Although licarin A presented an IC(50) for trypomastigotes higher than for epimastigotes, both substances acted as therapeutic trypanocidal agents, because they were able to kill parasites without affecting macrophages. Due to our results, burchellin and licarin A need to be further analysed to observe if they may be used as alternative blood additive prophylaxis against Chagas disease, since it has been established that blood transfusion is an important mechanism in the transmission process.

• Authors: M M O Cabral, J M Barbosa-Filho, G L A Maia, M C O Chaves, M V Braga, W De Souza, R O A Soares
• Journal: Experimental parasitology (Exp Parasitol) Vol. 124 Issue 3 Pg. 319-24 (Mar 2010) ISSN: 1090-2449 [Electronic] United States
• PMID: 19944690 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier Inc. All rights reserved.

Chemical References

• Benzofurans
• Lignans
• Plant Extracts
• Trypanocidal Agents
• licarin A
• burchellin

Topics

• Animals
• Benzofurans (chemistry, pharmacology, toxicity)
• Blood Transfusion (standards)
• Brazil
• Cell Survival (drug effects)
• Chagas Disease (prevention & control, transmission)
• Humans
• Inhibitory Concentration 50
• Lignans (chemistry, pharmacology, toxicity)
• Macrophages, Peritoneal (cytology, drug effects)
• Mice
• Mice, Inbred BALB C
• Microscopy, Electron, Transmission
• Plant Extracts (chemistry, pharmacology, toxicity)
• Trypanocidal Agents (chemistry, pharmacology, toxicity)
• Trypanosoma cruzi (drug effects, growth & development, ultrastructure)