Rodent rhabdomyosarcoma: comparison between total choline concentration at H-MRS and [18F]-fluoromethylcholine uptake at PET using accurate methods for collecting data.

Abstract

PURPOSE:

To compare choline concentration/amount at proton magnetic resonance spectroscopy (H-MRS) and [18F]-fluoromethylcholine (FCH) uptake at positron emission tomography (PET) in a tumour animal model.

PROCEDURES:

Twenty-two rats bearing grafted syngenic rhabdomyosarcoma in both thighs were examined on a 3T MR system and on a small animal PET system. Total choline concentration was measured on proton MR spectra using a so-called 'best internal fitting' volume of interest. Choline uptake was expressed as mean and maximum standardized uptake value (SUV and SUVmax, respectively) and as the percent of injected dose (%ID) after tumour delineation on fused PET-MR images. Data sets were displayed on standard scatter plots.

RESULTS:

Thirty-six tumours were available for analysis. The area under the curve of the 3.2 ppm choline peak ranged from 69 to 476 (mean, 192) in arbitrary units. Mean SUV values ranged from 0.05 to 0.49 (mean, 0.19) and the %ID from 0.05 to 2.28 (mean, 0.54). Scatter plots failed to reveal quantitative relationship between choline concentration and uptake. Empirically data-driven cut-off lines applied to choline amount (choline concentration x tumour volume) versus choline uptake suggested a paradoxically negative relationship.

CONCLUSION:

Total choline concentration did not correlate with FCH uptake in a tumour experimental model. A negative feedback of high values of total choline amount on cellular FCH uptake seemed to be present.

• Authors: Denis Rommel, Anne Bol, Jorge Abarca-Quinones, Frank Peeters, Annie Robert, Daniel Labar, Christine Galant, Vincent Gregoire, Thierry Duprez
• Journal: Molecular imaging and biology (Mol Imaging Biol) Vol. 12 Issue 4 Pg. 415-23 (Aug 2010) ISSN: 1860-2002 [Electronic] United States
• PMID: 19937391 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorine Radioisotopes
• fluoromethylcholine
• Choline

Topics

• Animals
• Choline (analogs & derivatives)
• Disease Models, Animal
• Fluorine Radioisotopes
• Magnetic Resonance Spectroscopy
• Positron-Emission Tomography (methods)
• Rats
• Rhabdomyosarcoma (diagnostic imaging, pathology, surgery)