Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in HIV-seropositive patients and gonococcal antimicrobial susceptibility: an update in Thailand.

Abstract

We surveyed the rate of chlamydial and gonococcal infections among human immunodeficiency virus (HIV)-seropositive patients in Thailand as well as the current status of antimicrobial resistance of Neisseria gonorrhoeae and determined the prevalence of penicillinase-producing N. gonorrhoeae (PPNG) in Thailand. A total of 1,158 endocervical swabs from 824 HIV-seropositive patients were collected to detect both organisms by Gen-Probe. The prevalences of chlamydial and gonococcal infection were 9.7 and 1.3%, respectively. Susceptibility of 122 gonococcal isolates to 6 drugs was determined by the disk diffusion method. None of the isolates was susceptible to penicillin or tetracycline. With respect to fluoroquinolones, more than 90% of the isolates were resistant to ciprofloxacin and ofloxacin. No gonococcal isolate with resistance to cefotaxime and ceftriaxone was detected. Among the 122 isolates, 83.6% or 102 isolates were PPNG, and most (79.5%) of these 122 isolates were further identified as PPNG plus tetracycline-resistant N. gonorrhoeae, with only 4.1% being PPNG alone. All of the 102 isolates identified as PPNG contained the bla(TEM) gene. We then performed a preliminary molecular study and identified, for the first time in Thailand, a PPNG isolate producing beta-lactamase and containing the bla(TEM) gene which was identical to the beta-lactamase TEM protein of Salmonella enterica identified as TEM-135.

• Authors: Somporn Srifeungfung, Anuvat Roongpisuthipong, Suvanna Asavapiriyanont, Rangsima Lolekha, Chanwit Tribuddharat, Somchai Lokpichart, Prasong Sungthong, Patrapee Tongtep
• Journal: Japanese journal of infectious diseases (Jpn J Infect Dis) Vol. 62 Issue 6 Pg. 467-70 (Nov 2009) ISSN: 1884-2836 [Electronic] Japan
• PMID: 19934542 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Bacterial Proteins
• Penicillinase

Topics

• Adolescent
• Adult
• Aged
• Anti-Bacterial Agents (pharmacology)
• Bacterial Proteins (genetics)
• Cervix Uteri (microbiology)
• Child
• Child, Preschool
• Chlamydia Infections (epidemiology)
• Chlamydia trachomatis (isolation & purification)
• Female
• Gonorrhea (epidemiology)
• HIV Infections (complications)
• Humans
• Infant
• Infant, Newborn
• Male
• Microbial Sensitivity Tests
• Middle Aged
• Neisseria gonorrhoeae (drug effects, enzymology, isolation & purification)
• Penicillinase (genetics)
• Prevalence
• Thailand (epidemiology)
• Young Adult