HFE genotype, parenchymal iron accumulation, and liver fibrosis in patients with nonalcoholic fatty liver disease.

Abstract

BACKGROUND & AIMS:

Mutations in the hemochromatosis gene (HFE) (C282Y and H63D) lead to parenchymal iron accumulation, hemochromatosis, and liver damage. We investigated whether these factors also contribute to the progression of fibrosis in patients with nonalcoholic fatty liver disease (NAFLD).

METHODS:

We studied clinical, histologic (liver biopsy samples for hepatocellular iron accumulation), serologic (iron and enzyme levels), and genetic (HFE genotype) data from 587 patients from Italy with NAFLD and 184 control subjects.

RESULTS:

Iron accumulation predominantly in hepatocyes was associated with a 1.7-fold higher risk of a fibrosis stage greater than 1 (95% confidence interval [CI]: 1.2-2.3), compared with the absence of siderosis (after adjustment for age, body mass index, glucose tolerance status, and alanine aminotransferase level). Nonparenchymal/mixed siderosis was not associated with moderate/severe fibrosis (odds ratio, 0.72; 95% CI: 0.50-1.01). Hepatocellular siderosis was more prevalent in patients with HFE mutations than in those without; approximately one third of patients with HFE mutations had parenchymal iron accumulation (range, 29.8%-35.7%, depending on HFE genotype). Predominantly hepatocellular iron accumulation occurred in 52.7% of cases of patients with HFE mutations. There was no significant association between either the presence of HFE mutations or specific HFE genotypes and the severity of liver fibrosis.

CONCLUSIONS:

Iron deposition predominantly in hepatocyes is associated with more severe liver damage in patients with NAFLD. However, HFE mutations cannot be used to identify patients with hepatocellular iron accumulation.

• Authors: Luca Valenti, Anna Ludovica Fracanzani, Elisabetta Bugianesi, Paola Dongiovanni, Enrico Galmozzi, Ester Vanni, Elena Canavesi, Ezio Lattuada, Giancarlo Roviaro, Giulio Marchesini, Silvia Fargion
• Journal: Gastroenterology (Gastroenterology) Vol. 138 Issue 3 Pg. 905-12 (Mar 2010) ISSN: 1528-0012 [Electronic] United States
• PMID: 19931264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 AGA Institute. Published by Elsevier Inc. All rights reserved.

Chemical References

• HFE protein, human
• Hemochromatosis Protein
• Histocompatibility Antigens Class I
• Membrane Proteins
• Iron

Topics

• Adult
• Biopsy
• Case-Control Studies
• Disease Progression
• Fatty Liver (etiology, genetics, metabolism, pathology)
• Female
• Gene Frequency
• Genetic Predisposition to Disease
• Hemochromatosis (complications, genetics, metabolism, pathology)
• Hemochromatosis Protein
• Histocompatibility Antigens Class I (genetics)
• Humans
• Iron (metabolism)
• Italy
• Liver (metabolism, pathology)
• Liver Cirrhosis (etiology, genetics, metabolism, pathology)
• Logistic Models
• Male
• Membrane Proteins (genetics)
• Middle Aged
• Mutation
• Odds Ratio
• Phenotype
• Risk Assessment
• Risk Factors
• Severity of Illness Index