Polysaccharides from Sargassum tenerrimum: structural features, chemical modification and anti-viral activity.

Abstract

Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry. Here, we exploit an approach to inhibiting HSV infection by using a sulfated fucoidan, and a guluronic acid-rich alginate derived from Sargassum tenerrimum, mimicking the active domain of the entry receptor. These macromolecules have apparent molecular masses of 30+/-5 and 26+/-5 kDa, respectively. They and their chemically sulfated derivatives showed activity against herpes simplex virus type 1 (HSV-1). Their inhibitory concentration 50% (IC(50)) values were in the range 0.5-15 microg/ml and they lacked cytotoxicity at concentrations up to 1000 microg/ml. The anti-HSV activity increased with increasing sulfate ester content. Our results suggest the feasibility of inhibiting HSV infection by blocking viral entry with polysaccharide having specific structure.

• Authors: Sharmistha Sinha, Akram Astani, Tuhin Ghosh, Paul Schnitzler, Bimalendu Ray
• Journal: Phytochemistry (Phytochemistry) Vol. 71 Issue 2-3 Pg. 235-42 (Feb 2010) ISSN: 1873-3700 [Electronic] England
• PMID: 19931103 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2009 Elsevier Ltd. All rights reserved.

Chemical References

• Alginates
• Antiviral Agents
• Hexuronic Acids
• Plant Extracts
• Polysaccharides
• guluronic acid
• Glucuronic Acid
• fucoidan

Topics

• Alginates (chemistry, pharmacology, therapeutic use)
• Animals
• Antiviral Agents (chemistry, pharmacology, therapeutic use)
• Cells, Cultured
• Glucuronic Acid (chemistry, pharmacology, therapeutic use)
• Haplorhini
• Herpes Simplex (drug therapy, virology)
• Herpesvirus 1, Human (drug effects)
• Hexuronic Acids (chemistry, pharmacology, therapeutic use)
• Inhibitory Concentration 50
• Phytotherapy
• Plant Extracts (chemistry, pharmacology, therapeutic use)
• Polysaccharides (chemistry, pharmacology, therapeutic use)
• Sargassum (chemistry)
• Structure-Activity Relationship
• Virus Integration (drug effects)