Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals for magnetically triggered delivery of siRNA.

Abstract

Pluronic/polyethylenimine shell crosslinked nanocapsules with embedded magnetite nanocrystals (PPMCs) were developed for magnetically triggered delivery of siRNA. The positively charged PPMCs formed stable nanosized polyelectrolyte complexes via electrostatic interactions with negatively charged siRNA-polyethylene glycol conjugate (siRNA-s-s-PEG) that was linked via a cleavable disulfide linkage. PPMC/siRNA-s-s-PEG polyelectrolyte complexes were efficiently taken up by cancer cells upon exposure to a magnet, thereby enhancing intracellular uptake and silencing effect of siRNA. The present study suggests that these novel nanomaterials could be potentially utilized for magnetically triggered delivery of various nucleic acid-based therapeutic agents.

• Authors: Kyuri Lee, Ki Hyun Bae, Yuhan Lee, Soo Hyeon Lee, Cheol-Hee Ahn, Tae Gwan Park
• Journal: Macromolecular bioscience (Macromol Biosci) Vol. 10 Issue 3 Pg. 239-45 (Mar 10 2010) ISSN: 1616-5195 [Electronic] Germany
• PMID: 19924685 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cross-Linking Reagents
• Nanocapsules
• RNA, Small Interfering
• Poloxamer
• Green Fluorescent Proteins
• Polyethyleneimine
• Fluorescein
• Ferrosoferric Oxide

Topics

• Cell Line, Tumor
• Cross-Linking Reagents (pharmacology)
• Drug Delivery Systems (methods)
• Ferrosoferric Oxide (chemistry)
• Fluorescein
• Green Fluorescent Proteins (metabolism)
• Humans
• Intracellular Space (drug effects, metabolism)
• Magnetics (methods)
• Microscopy, Confocal
• Nanocapsules (chemistry, ultrastructure)
• Particle Size
• Poloxamer (pharmacology)
• Polyethyleneimine (pharmacology)
• RNA, Small Interfering (metabolism)