Cerebrospinal fluid (CSF) is considered as the most promising body fluid target for the discovery of
biomarkers for early diagnosis of
neurodegenerative diseases such as
Creutzfeldt-Jakob disease in humans and
bovine spongiform encephalopathy in cattle. For the recognition of disease-associated changes in bovine CSF
protein patterns, a detailed knowledge of this
proteome is a prerequisite. The absence of a high-resolution CSF
proteome map prompted us to determine all bovine CSF
protein spots that can be visualised on 2-D
protein gels. Using state-of-the-art 2-DE technology for
proteome mapping of bovine ante mortem CSF combined with sensitive fluorescent
protein staining and MALDI-TOF/TOF MS for
protein identification, a highly detailed 2-DE map of the bovine CSF
proteome was established. Besides the
proteins mapped by earlier studies, this map contains 66 different
proteins, including 58 which were not annotated in bovine 2-DE CSF maps before.