Detection of polyunsaturated omega-6 fatty acid in human malignant prostate tissue by 1D and 2D high-resolution magic angle spinning NMR spectroscopy.

Abstract

OBJECT:

Polyunsaturated omega-6 fatty acids (PUFAs) have been shown to promote prostate cancer. Here, we describe the use of HRMAS NMR spectroscopy to detect omega-6 PUFA species in prostate tissues.

MATERIALS AND METHODS:

Samples originating from non-malignant (n = 54) and malignant (n = 27) prostate tissues (from 27 prostatectomized men) were studied by 1D (1)H, 2D (1)H-(1)H and (1)H-(13)C HRMAS NMR spectroscopy followed by histopathological characterization.

RESULTS:

HRMAS NMR proved to be a powerful, non-destructive method to identify and characterize PUFAs. The omega-6 PUFA was found in 15% of examined human prostate tumors.

CONCLUSION:

It is possible to detect PUFAs in prostate tissues using our NMR-based spectroscopic approach.

• Authors: Katarina Stenman, Jón B Hauksson, Gerhard Gröbner, Pär Stattin, Anders Bergh, Katrine Riklund
• Journal: Magma (New York, N.Y.) (MAGMA) Vol. 22 Issue 6 Pg. 327-31 (Dec 2009) ISSN: 1352-8661 [Electronic] Germany
• PMID: 19921294 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Fatty Acids, Omega-6
• Spin Labels

Topics

• Biomarkers, Tumor (analysis, metabolism)
• Diagnostic Imaging
• Fatty Acids, Omega-6 (analysis)
• Humans
• Magnetic Resonance Spectroscopy
• Male
• Medical Oncology (methods)
• Prostate (metabolism, pathology)
• Prostatic Neoplasms (diagnosis, metabolism, pathology)
• Sensitivity and Specificity
• Spin Labels