Hypoxia protects neuronal cells from human prion protein fragment-induced apoptosis.

Abstract

Prion diseases are neurodegenerative disorders characterized by the accumulation of an abnormal isoform of the prion protein PrP(Sc). Human prion protein fragment, PrP (106-126) (prion protein peptide 106-126), may contain most of the pathological features associated with PrP(Sc). Hypoxic conditions elicit cellular responses adaptively designed to improve cell survival and have an important role in the process of cell survival. We investigate the effects of hypoxia on PrP (106-126)-induced apoptosis in the present study. Human neuroblastoma and glioblastoma cells were incubated with varied doses of PrP (106-126) under both normoxic or hypoxic conditions, in order to determine the regulatory effects of hypoxia on PrP (106-126)-induced apoptosis. The results indicate that hypoxia protects neuronal cells against PrP (106-126)-induced cell death by activating the Akt signal, which is inactivated by prion proteins, and inhibiting PrP (106-126)-induced caspase 3 activation. Low oxygen conditions increase the Bcl-2 protein, which is associated with anti-apoptotic signals, and recover the PrP (106-126)-induced reduction in mitochondrial transmembrane potential. This study demonstrates that hypoxia inhibits PrP (106-126)-induced neuron cell death by regulating Akt and Akt-related signaling, and it also suggests that prion-related neuronal damage and disease may be regulated by hypoxia or by hypoxic-inducing genes.

• Authors: Jae-Suk Seo, Jae-Won Seol, Myung-Hee Moon, Jae-Kyo Jeong, You-Jin Lee, Sang-Youel Park
• Journal: Journal of neurochemistry (J Neurochem) Vol. 112 Issue 3 Pg. 715-22 (Feb 2010) ISSN: 1471-4159 [Electronic] England
• PMID: 19919574 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• Peptide Fragments
• Prions
• Proto-Oncogene Proteins c-bcl-2
• Tetrazolium Salts
• Thiazoles
• prion protein (106-126)
• Oncogene Protein v-akt
• Caspase 3
• thiazolyl blue

Topics

• Analysis of Variance
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Hypoxia
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• In Situ Nick-End Labeling (methods)
• Membrane Potential, Mitochondrial (drug effects)
• Neuroblastoma (pathology)
• Oncogene Protein v-akt (metabolism)
• Peptide Fragments (pharmacology)
• Prions (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Tetrazolium Salts
• Thiazoles