Abstract | OBJECTIVE: METHODS: RESULTS: We successfully generated seven MPO-specific T-cell clones, five from the patients and two from healthy donors. Two clones recognized the light chain of MPO and five recognized the heavy chain. All the clones were HLA-DR-restricted CD4(+)CD8(-) helper T cells. The T-cell clones shared TCR Beta CDR3 amino acid motifs, depending on their MPO epitope: AGXiXiN was used by clones recognizing the light chain and TGXiS or QGXiE by those recognizing the heavy chain, whether the cells were derived from MPA patients or healthy subjects. However, the cytokine expression profiles of the patients' clones were skewed towards the Th1 phenotype, whereas the healthy individuals' clones remained Th0. CONCLUSIONS: We have characterized MPO-reactive T cells in detail. This information may be useful for elucidating the mechanism of ANCA production and for developing selective therapeutic strategies for MPO- ANCA-associated vasculitis.
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Authors | N Seta, S Kobayashi, H Hashimoto, M Kuwana |
Journal | Clinical and experimental rheumatology
(Clin Exp Rheumatol)
2009 Sep-Oct
Vol. 27
Issue 5
Pg. 826-9
ISSN: 0392-856X [Print] Italy |
PMID | 19917167
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantigens
- Cytokines
- Receptors, Antigen, T-Cell
- Peroxidase
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Topics |
- Autoantigens
(immunology)
- Autoimmunity
- Case-Control Studies
- Cell Line
- Cytokines
(metabolism)
- Humans
- Microscopic Polyangiitis
(immunology)
- Peroxidase
(immunology)
- Receptors, Antigen, T-Cell
(immunology)
- T-Lymphocytes, Helper-Inducer
(immunology, metabolism)
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