Abstract | BACKGROUND: Drug resistance is a primary hindrance for the efficiency of chemotherapy against osteosarcoma. Although chemotherapy has improved the prognosis of osteosarcoma patients dramatically after introduction of neo-adjuvant therapy in the early 1980's, the outcome has since reached plateau at approximately 70% for 5 year survival. The remaining 30% of the patients eventually develop resistance to multiple types of chemotherapy. In order to overcome both the dose-limiting side effects of conventional chemotherapeutic agents and the therapeutic failure incurred from multidrug resistant (MDR) tumor cells, we explored the possibility of loading doxorubicin onto biocompatible, lipid-modified dextran-based polymeric nanoparticles and evaluated the efficacy. METHODS:
Doxorubicin was loaded onto a lipid-modified dextran based polymeric nano-system. The effect of various concentrations of doxorubicin alone or nanoparticle loaded doxorubicin on KHOS, KHOSR2, U-2OS, and U-2OSR2 cells was analyzed. Effects on drug retention, immunofluorescence, Pgp expression, and induction of apoptosis were also analyzed. RESULTS: CONCLUSION:
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Authors | Michiro Susa, Arun K Iyer, Keinosuke Ryu, Francis J Hornicek, Henry Mankin, Mansoor M Amiji, Zhenfeng Duan |
Journal | BMC cancer
(BMC Cancer)
Vol. 9
Pg. 399
(Nov 16 2009)
ISSN: 1471-2407 [Electronic] England |
PMID | 19917123
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Dextrans
- Drug Carriers
- Polymers
- Doxorubicin
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Topics |
- Antineoplastic Agents
(administration & dosage)
- Apoptosis
(drug effects)
- Blotting, Western
- Bone Neoplasms
(drug therapy)
- Cell Line, Tumor
- Dextrans
(pharmacology)
- Doxorubicin
(administration & dosage)
- Drug Carriers
(pharmacology)
- Drug Delivery Systems
(methods)
- Drug Resistance, Neoplasm
(drug effects)
- Flow Cytometry
- Humans
- Microscopy, Fluorescence
- Nanoparticles
(therapeutic use)
- Osteosarcoma
(drug therapy)
- Polymers
(pharmacokinetics)
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