IL-9 regulates pathology during primary and memory responses to respiratory syncytial virus infection.
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| Abstract |
IL-9 is a cytokine of great current interest associated with allergic/Th2 responses. High levels of IL-9 are present in bronchial secretions from infants with respiratory syncytial virus (RSV) bronchiolitis. To test its effects in RSV disease with a Th2 profile, BALB/c mice were vaccinated with recombinant vaccinia virus expressing the RSV G protein. On RSV challenge, immunized mice developed augmented disease characterized by enhanced pulmonary Th2 and local IL-9 production peaking on days 7-10 of RSV infection. Depletion with anti-IL-9 Ab at vaccination or RSV challenge enhanced viral clearance. Depletion only at challenge had no effect on disease severity, whereas depletion at immunization and challenge enhanced Th1 responses, inhibited virus-specific IgG1 production, and enhanced disease severity. By contrast, depletion of IL-9 at immunization boosted IgG2a and inhibited the Th2 response and disease during subsequent infection without a concomitant increase in type 1 cytokines. Adoptive transfer of secondary memory CD4 T cells from the spleens of IL-9-depleted mice into naive recipients replicated many of the effects of depletion, indicating that IL-9 acts via CD4 T cells. Therefore, IL-9 is a previously unknown but key modulator of antiviral immunity, regulating T and B cell responses and having potent and specific effects on viral lung disease.
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| Authors | Jonathan S Dodd, Eda Lum, John Goulding, Roshell Muir, Jacques Van Snick, Peter J M Openshaw |
| Journal | Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 183 Issue 11 Pg. 7006-13 (Dec 01 2009) ISSN: 1550-6606 [Electronic] United States |
| PMID | 19915054 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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