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Human ribosomal protein S13 promotes gastric cancer growth through down-regulating p27(Kip1).

Abstract
Our previous works revealed that human ribosomal protein S13 (RPS13) was up-regulated in multidrug-resistant gastric cancer cells and overexpression of RPS13 could protect gastric cancer cells from drug-induced apoptosis. The present study was designed to explore the role of RPS13 in tumorigenesis and development of gastric cancer. The expression of RPS13 in gastric cancer tissues and normal gastric mucosa was evaluated by immunohistochemical staining and Western blot analysis. It was found RPS13 was expressed at a higher level in gastric cancer tissues than that in normal gastric mucosa. RPS13 was then genetically overexpressed in gastric cancer cells or knocked down by RNA interference. It was demonstrated that up-regulation of RPS13 accelerated the growth, enhanced in vitro colony forming and soft agar cologenic ability and promoted in vivo tumour formation potential of gastric cancer cells. Meanwhile, down-regulation of RPS13 in gastric cancer cells resulted in complete opposite effects. Moreover, overexpression of RPS13 could promote G1 to S phase transition whereas knocking down of RPS13 led to G1 arrest of gastric cancer cells. It was further demonstrated that RPS13 down-regulated p27(kip1) expression and CDK2 kinase activity but did not change the expression of cyclin D, cyclin E, CDK2, CDK4 and p16(INK4A). Taken together, these data indicate that RPS13 could promote the growth and cell cycle progression of gastric cancer cells at least through inhibiting p27(kip1) expression.
AuthorsXueyan Guo, Yongquan Shi, Yawen Gou, Jipeng Li, Shuang Han, Yanqi Zhang, Jianhua Huo, Xiaoxuan Ning, Li Sun, Yu Chen, Shiren Sun, Daiming Fan
JournalJournal of cellular and molecular medicine (J Cell Mol Med) Vol. 15 Issue 2 Pg. 296-306 (Feb 2011) ISSN: 1582-4934 [Electronic] England
PMID19912438 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
Chemical References
  • Cyclin D
  • Cyclin E
  • Cyclin-Dependent Kinase Inhibitor p16
  • RPS13 protein, human
  • Ribosomal Proteins
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinase 4
Topics
  • Animals
  • Apoptosis
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin D (biosynthesis)
  • Cyclin E (biosynthesis)
  • Cyclin-Dependent Kinase 2 (biosynthesis, metabolism)
  • Cyclin-Dependent Kinase 4 (biosynthesis)
  • Cyclin-Dependent Kinase Inhibitor p16 (biosynthesis)
  • Cyclin-Dependent Kinase Inhibitor p27 (biosynthesis, genetics, metabolism)
  • Down-Regulation
  • G1 Phase Cell Cycle Checkpoints
  • Gastric Mucosa (metabolism)
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • RNA Interference
  • Ribosomal Proteins (biosynthesis, metabolism)
  • Stomach Neoplasms (metabolism, pathology)

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