Abstract |
The high tumor uptake of ultrasmall near-infrared quantum dots (QDs) attributed to the enhanced permeability and retention effect is reported. InAs/InP/ZnSe QDs coated by mercaptopropionic acid (MPA) exhibit an emission wavelength of about 800 nm (QD800-MPA) with very small hydrodynamic diameter (<10 nm). Using 22B and LS174T tumor xenograft models, in vivo and ex vivo imaging studies show that QD800-MPA is highly accumulated in the tumor area, which is very promising for tumor detection in living mice. The ex vivo elemental analysis ( Indium) using inductively coupled plasma (ICP) spectrometry confirm the tumor uptake of QDs. The ICP data are consistent with the in vivo and ex vivo fluorescence imaging. Human serum albumin (HSA)-coated QD800-MPA nanoparticles (QD800-MPA-HSA) show reduced localization in mononuclear phagocytic system-related organs over QD800-MPA plausibly due to the low uptake of QD800-MPA-HSA in macrophage cells. QD800-MPA-HSA may have great potential for in vivo fluorescence imaging.
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Authors | Jinhao Gao, Kai Chen, Renguo Xie, Jin Xie, Seulki Lee, Zhen Cheng, Xiaogang Peng, Xiaoyuan Chen |
Journal | Small (Weinheim an der Bergstrasse, Germany)
(Small)
Vol. 6
Issue 2
Pg. 256-61
(Jan 2010)
ISSN: 1613-6829 [Electronic] Germany |
PMID | 19911392
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Lectins
- Cadmium
- 3-Mercaptopropionic Acid
- Fluorescein-5-isothiocyanate
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Topics |
- 3-Mercaptopropionic Acid
(chemistry)
- Animals
- Cadmium
(chemistry)
- Diagnostic Imaging
(methods)
- Female
- Fluorescein-5-isothiocyanate
(metabolism)
- Humans
- Lectins
(metabolism)
- Mice
- Mice, Nude
- Nanoparticles
(chemistry)
- Neoplasms
(pathology)
- Organ Specificity
- Quantum Dots
- Spectroscopy, Near-Infrared
(methods)
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