TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells.

Abstract	The androgen receptor (AR) is a ligand-dependent transcription factor that belongs to the family of nuclear receptors, and its activity is regulated by numerous AR coregulators. AR plays an important role in prostate development and cancer. In this study, we found that TRIM24/transcriptional intermediary factor 1alpha (TIF1alpha), which is known as a ligand-dependent nuclear receptor co-regulator, interacts with AR and enhances transcriptional activity of AR by dihydrotestosterone in prostate cancer cells. We showed that TRIM24 functionally interacts with TIP60, which acts as a coactivator of AR and synergizes with TIP60 in the transactivation of AR. We also showed that TRIM24 binds to bromodomain containing 7 (BRD7), which can negatively regulate cell proliferation and growth. A luciferase assay indicated that BRD7 represses the AR transactivation activity upregulated by TRIM24. These findings indicate that TRIM24 regulates AR-mediated transcription in collaboration with TIP60 and BRD7.
Authors	Misato Kikuchi, Fumihiko Okumura, Tadasuke Tsukiyama, Masashi Watanabe, Naoto Miyajima, Junji Tanaka, Masahiro Imamura, Shigetsugu Hatakeyama
Journal	Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1793 Issue 12 Pg. 1828-36 (Dec 2009) ISSN: 0006-3002 [Print] Netherlands
PMID	19909775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Androgens BRD7 protein, human Brd7 protein, mouse Chromosomal Proteins, Non-Histone Ligands Neoplasm Proteins Nuclear Proteins Receptors, Androgen Repressor Proteins Trans-Activators Transcription Factors transcriptional intermediary factor 1 Dihydrotestosterone Histone Acetyltransferases KAT5 protein, human Kat5 protein, mouse Lysine Acetyltransferase 5
Topics	Androgens (pharmacology) Animals COS Cells Cell Line, Tumor Chlorocebus aethiops Chromosomal Proteins, Non-Histone (genetics, metabolism) Dihydrotestosterone (pharmacology) Histone Acetyltransferases (genetics, metabolism) Humans Ligands Lysine Acetyltransferase 5 Male Mice Neoplasm Proteins (genetics, metabolism) Nuclear Proteins (genetics, metabolism) Prostatic Neoplasms (genetics, metabolism) Receptors, Androgen (genetics, metabolism) Repressor Proteins (genetics, metabolism) Trans-Activators Transcription Factors (genetics, metabolism) Transcription, Genetic

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