Widespread expression of prostate apoptosis response-4 in nasopharyngeal carcinoma.

Abstract

BACKGROUND:

Prostate apoptosis response-4 (Par-4) augments apoptosis in various tumors, either during apoptotic insult or by ectopic overexpression. However, investigation of Par-4 expression in nasopharyngeal carcinoma (NPC) is lacking.

METHODS:

Specimens from patients with NPC, hypopharyngeal carcinoma (HPC), or oral cavity cancer were examined for Par-4 expression using immunohistochemistry. NPC cell proliferation and apoptosis were analyzed using immunohistochemical staining for Ki67, B-cell lymphoma 2 (Bcl-2), and in situ terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick end-labeling (TUNEL) assay, respectively.

RESULTS:

Par-4 was ubiquitously expressed in NPC biopsies (96.2%, 25/26) and was significantly higher than in HPC (47.6%, 50/105, p < .0001) and oral cavity cancers (38.7%, 12/31, p < .0001). Remarkably, apoptosis of NPC cells was absent and Par-4 expression was associated with obvious expression of Bcl-2 and Ki67 in all patients tested with NPC.

CONCLUSIONS:

Immunohistochemistry results showed widespread expression of Par-4 in NPC and revealed sustainable proliferation of NPC cells regardless of Par-4 expression.

• Authors: Jeng-Woei Lee, Wei-Ting Hsiao, Kuei-Fang Lee, Lai-Fa Sheu, Hsue-Yin Hsu, Lee-Ping Hsu, Borcherng Su, Moon-Sing Lee, Yih-Chih Hsu, Chung-Hsing Chang
• Journal: Head & neck (Head Neck) Vol. 32 Issue 7 Pg. 877-85 (Jul 2010) ISSN: 1097-0347 [Electronic] United States
• PMID: 19908316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: .(c) 2009 Wiley Periodicals, Inc.

Chemical References

• Apoptosis Regulatory Proteins
• Ki-67 Antigen
• Proto-Oncogene Proteins c-bcl-2
• prostate apoptosis response-4 protein

Topics

• Apoptosis
• Apoptosis Regulatory Proteins (metabolism)
• Carcinoma (metabolism, pathology)
• Cell Proliferation
• Cohort Studies
• Female
• Humans
• Hypopharyngeal Neoplasms (metabolism, pathology)
• Ki-67 Antigen (metabolism)
• Male
• Mouth Neoplasms (metabolism, pathology)
• Nasopharyngeal Neoplasms (metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)